IMPACT OF SOLVENT TYPES ON ANTIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CAESARIA TOMENTOSA
- Vol.11(4)
- M P Innovare Academic Sciences Pvt Ltd 2019
- 33-38p.
Objective: The study deals with the antimicrobial activity of five solvent extracts of roots of Caesaria tomentosa against seven microorganisms: two gram-positive ( Staphylococcus aureus and Bacillus subtilis), three gram-negative bacteria ( Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimrium) and two fungal strains (Candida albicans and Aspergillus niger). Methods: Roots of C. tomentosa were extracted with different solvents(n-hexane, et hanol, chloroform, acetone and water) and were subj ected to antibacterial as well as antifungal screening by We ll Diffusion Method. The Minimum Inhibitory Concent ration(MIC) was also performed by two-fold dilution . Results: The maximum inhibition zone at 50μg/ml concentratio n of n-hexane was 32 mm. These indicate that some a ctive substances in C. tomentosa dissolved in varying degrees in the five solvents. The MIC for n-hexane was 1.6 μg/ml for S. aureus leading to a conclusion that the n- hexane extract was found to be the most potent. Conclusion: All extracts were very effective against S. aureus. As for the solvents, the n-hexane extract had the best inhibitory effect among five solvents tested.