Rajesh, R. <br/><br/>
VALIDATED RP -HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PYRANTEL  PAMOATE AND PRAZIQUANTEL  IN BULK AND PHARMACEUTICAL DOSAGE FORM 

 - Vol.1(5) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 62-67p. 
<br/><br/> Objective: <br/>To develop a simple, accurate and precise <br/>reverse-<br/>phase high<br/>-performance liquid chromatography (<br/>RP<br/>-HPLC<br/>) metho<br/>d and subsequently <br/>validate <br/>for the simultaneous estimation of praziquantel (PZ<br/>Q) and pyrantel pamoate (PP) in the <br/>pharmaceutical dosage form.<br/>Method<br/>s: <br/>The  chromatographic  separation  was  achieved  on  Phenomenex  Luna  C<br/>18<br/>Result<br/>s: <br/>The retention time of PZQ and PP was found to be 3.897 min and 1.697 min re<br/>spectively. The method was validated in terms of specificity, <br/>accuracy, precision, linearity and robustness as per ICH guidelines. Linearity was obtained in the concentration range of<br/> 20<br/>–<br/>60 μg<br/>/ml<br/> for both PZQ <br/>and PP with correlation coefficients of 0.987 and 0.998 respectively. The accuracy of the method was determined using a recov<br/>ery test and found as <br/>98.44 % to 100.35 %. All parameters are found to be within the acceptable limit.<br/>  column  (250  mm  ×  4.6  mm,  5  <br/>μm) as st<br/>ationary  phase  <br/>maintained at an ambient temperature with a mobile phase comprising of wate<br/>r: <br/>acetonitrile (20<br/>: 80) at a flow rate of 1.0 ml/min and UV detection <br/>at 220 nm.<br/>Conclu<br/>sio<br/>n: <br/>The developed RP<br/>-HPLC method was simple, rapid, accurate, precise for the simultaneous estimation of PZQ and PP in bulk and tablet <br/>dosage form 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS