Ibrahim Siddig Hamid <br/><br/>
IN VITRO  SUSCEPTIBILITY OF ISOLATED  SHIGELLA FLEXNERI  AND  SHIGELLA DYSENTERIAE  TO THE ETHANOLIC EXTRACTS OF  TRACHYSPERMUM AMMI  AND  PEGANUM HARMALA 

 - Vol.11(1) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 103-106p. 
<br/><br/> Objective: <br/>This  study  represents  the  first  attempt  to  investigate  the  antimicrobial  activity  of  <br/>Peganum <br/>harmala<br/>, <br/>and<br/>Trachyspermum <br/>ammi<br/>  seeds <br/>extract<br/>against the isolated bacillary dysentery<br/>-causing<br/> microorganisms.<br/>Method<br/>s: <br/>T. <br/>ammi<br/>  and <br/>P. <br/>harmala<br/>  were  extracted  by  96%  ethanol  using  Soxhlet  apparatus.  The  extracts  were  screened  for  their  phytochemical  <br/>constituents. Their antimicrobial activity against <br/>the isolated<br/> dysentery<br/>-causing<br/> microorganisms was evaluated using the agar diffusion method. <br/>Result<br/>s: <br/>The  antimicrobial  activity  result  showed  that,  the  two  isolated  bacteria,  <br/>Shigella  flexneri<br/>, <br/>and<br/>  Shigella  dysenteriae<br/>  were  found  to  be  <br/>sensitive  to  the  extract  of  <br/>T. <br/>ammi<br/>seed  with  <br/>inhibition  zones  up  to  2<br/>5  mm<br/>,  compared  to  the  inhibition  zone  of  2<br/>0  mm<br/>  produced  by  Gentamycin  <br/>standard drug<br/>, this is mainly due to the presence of the different phytochemical in the extract such as tannin, flavonoids, terpenoids whi<br/>ch are well <br/>known  for  th<br/>eir  antimicrobial  effects<br/>.  The  two  isolated  bacteria  were <br/>found  to  be  insensitive  (zero  mm)  to  <br/>P. <br/>harmala<br/>extract, <br/>Amoxicillin<br/>,  and<br/>Amoclan  (Amoxicillin<br/>+clavulanic  acid)  standard  <br/>drugs,<br/>  this  is  due  to  the  fact  that,  the  phytochemicals  constituents  of<br/>P. <br/>harmala<br/>  possess <br/>the <br/>antagonistic effect to <br/>each <br/>other’s<br/>. Addition t<br/>o; <br/>these bacteria became resistant to both Amoxicillin and Amoclan.<br/>Conclusio<br/>n: <br/>From  the  results  it  concludes,  <br/>T. <br/>ammi<br/>  seeds  extract  had  a  considerable  level  of  antimicrobial  activity  against  bacillary  dysentery<br/>-<br/>causing<br/> microorganisms resistant to Amoxicillin and Amoxicillin<br/>+clavulanic acid drugs.  
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS