NEW VALIDATED METHOD DEVELOPMENT FOR THE ESTIMATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM IN BULK FORM BY VISIBLE SPECTROSCOPY
- Vol.10(12)
- M P Innovare Academic Sciences Pvt Ltd 2018
- 50-57p.
Objective: To develop three novel , sensitive, simple validated visible spectrophotometric methods for the quantitative estimation of sulfamethoxazole (SMZ) and trimethoprim (TMP) in bulk form . Method s: Methods were based on coupling the d iazotized aromatic primary amino group of the studied drugs with o- phenylenediamine (OPD ) in an acidic medium . The first two methods have been proposed for estimation of SMZ and rest for TMP . The resulting products were m easured by spectrophotometric (m ethod I, II and III) tools. The methods we re validated as per ICH guidelines . Result s: In method I, the absorbance was measured at 482 and 457 nm with linearity ranges of 4.0 -40.0 and 5.0 -45.0 μg /ml for SMZ . On the other hand, method III was devoted to estimate TMP spectrophotometrically at 457 nm with linearity range of 5 - 30 μg /ml . The r 2 Conclusio n: The study concludes that visible spectrophotometric validation method s can be very efficien t and economically promising technique for the quantitative analysis of S MZ and TMP in bulk form . The statistical analysis of data indicates that the developed methods were reproducible and specific. It was found that there is a good agreement between the obtained results and those obtained by the reported method s; moreover they can be used for the routine estimations of SMZ and TMP in bulk form