Abobakr, Fatma E. <br/><br/>
FORMULATION AND OPTIMIZATION OF  TERBINAFINE  HCl SOLID  LIPID NANOPARTICLES FOR TOPICAL ANTIFUNGAL ACTIVITY 

 - Vol.11(12) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 16-25p. 
<br/><br/> Objective: <br/>Soli<br/>d lipid nanoparticles (SLNs) are<br/> at the forefront <br/>of the rapidly developing field of nanotechnology with several potential applications <br/>in  drug  delivery  and  res<br/>earch.<br/>  The  aim  of  this  study  was<br/>  to <br/>develop  and  characterize  SLNs  formulae  of  Terbinafine  HCl<br/>  (TFH)  for<br/>  topical  drug  <br/>delivery <br/>application<br/>s. <br/>Method<br/>s: <br/>SLNs  were  prepared  using  the  solvent <br/>injection  technique.  Glyceryl  M<br/>onostearate  (GMS)  served  as  the  lipid  base.<br/>  Three <br/>stabilizer<br/>s; <br/>Tween 80, C<br/>remophor<br/> RH40<br/>, and <br/>Poloxame<br/>r 188<br/>, were used. The effect of stabilizer<br/> type and <br/>concentration<br/>, as well<br/> as<br/> the li<br/>pid concentration<br/>, were <br/>studied<br/>,  factorial  design  of <br/>3<br/>2<br/>*2<br/>1<br/>was  applied.<br/>  The  prepared  SLNs  were  characterized<br/>  regarding <br/>their <br/>particle  size,  zeta  potential,  polydispersity  <br/>index<br/>  (PDI)<br/>,  entrapment  eff<br/>iciency <br/>percent <br/>(EE  %)<br/>,  and  physicochemical<br/>  stability.<br/>  The <br/>selected  formulae  were  subjected  to  further  investigations<br/>such as morphological studies, <br/>in  vitro<br/> release studies<br/>, and Infrared (IR) spectroscopy. <br/>The<br/>y were compared with<br/> the marketed<br/> cream <br/>Lamifen<br/>®<br/> in <br/>term of their antifungal activity<br/> against <br/>Candida alb<br/>icans<br/>. <br/>Result<br/>s: <br/>Lipid  concentration<br/>,  together  with  the  type  and  concentration  of  stabilizer<br/>,  appeared  to  be<br/>  the  main  cornerstones  <br/>which<br/>  affect  the  <br/>formation of SLNs. Smaller particle size was observed <br/>when<br/> increasing the stabilizer concentration<br/> and decrea<br/>sing the lipid concentration. Higher <br/>EE% was observed <br/>when<br/> increasing both the stabilizer and the lipid concentration<br/>s. Formulae (F6, F12 andF19) were selected as the most suitable <br/>SLNs<br/> with<br/> optimum particle size of 480.2<br/>±18.89, 458.6<br/>±12.45 <br/>and<br/> 246.7<br/>±10.5 <br/>nm<br/>, respectively as well as the highest EE% of 87.13<br/>±0.19, 93.69<br/>±0.7 <br/>and<br/>  95.06<br/>±0.25<br/>,  respectivel<br/>y. <br/>In  vitro<br/>  microbiological<br/>  screening  of  <br/>their <br/>antifungal  activity  showed  significantly  larger <br/>zone<br/>s  of  inhibition  <br/>of <br/>diameters  25.9<br/>±0.25, <br/>25<br/>±0.35 <br/>and<br/>  24.67<br/>±0.3<br/>6  mm<br/>,  respectively<br/>  in  comparison  with  the  marketed<br/>  Lamifen®<br/>  cream<br/>  which  showed  a  zone  of  <br/>11.2<br/>±0.44 mm diameter<br/>.<br/>Conclusio<br/>n: <br/>Applying<br/>  SLNs <br/>containing  TFH<br/>  as  topic 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS