ALNAQBI, A. M. S. <br/><br/>
New Validated Diffuse Reflectance Infrared Fourier Transform Spectroscopic Method for the Quantification of Levofloxacin in Pharmaceutical Dosage Form 

 - Vol.83(3), May-June 
 - Mumbai  Indian Journal of Pharmaceutical Science  2021 
 - 430-436p. 
<br/><br/> Levofloxacin is a synthetic fluoroquinolone antibiotic available in different dosage forms for the treatment<br/>of various bacterial infections. Here in, we report the validated diffuse reflectance infrared Fourier<br/>transform spectroscopic method for quantitative analysis of levofloxacin in the solid dosage forms. This<br/>method offers the advantage of being eco-friendly that is applied for solid state samples. Appropriate<br/>quantities of levofloxacin and dry potassium bromide were mixed to get the desired concentration of samples<br/>for Fourier transform infrared spectroscopic analysis using a diffuse reflectance sampling interface. The<br/>peak in the Fourier transform infrared spectrum around 1724 cm-1 was observed for the carbonyl group<br/>of levofloxacin. The calibration curve was plotted for the area under the curve of carbonyl peak against<br/>the concentration of calibration standards. A linear calibration curve with a correlation coefficient of 0.999<br/>was obtained in the range of 0.3-1.8 % w/w of levofloxacin. The calibration curve method was linear,<br/>precise and accurate as per guidelines. The method employed for quantification of levofloxacin in three<br/>marketed tablet dosage forms and laboratory physical mixture yielded a mean recovery of 101.32 and<br/>98.11 percent by weight, respectively. Overall, the precision, accuracy and reproducibility of the proposed<br/>diffuse reflectance infrared Fourier transform spectroscopy method make it appropriate for quantitative<br/>analysis of levofloxacin in solid dosage forms. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS