BUKKAPATNAM, V. <br/><br/>
Experimental Design Methodology for Simultaneous Determination of Anti-Diabetic Drugs by Reverse Phase Liquid Chromatographic Method 

 - Vol.83(3), May-June 
 - Mumbai  Indian Journal of Pharmaceutical Science  2021 
 - 523-534p. 
<br/><br/> A liquid chromatographic method has been established for the separation of metformin, glipizide,<br/>gliclazide, glibenclamide and glimepiride using experimental design. The main intention of this method is<br/>to optimize suitable chromatographic conditions for the proper elution of the drug molecules with minimal<br/>analysis time. The relationship between the individual and combined effect of critical process parameters<br/>and chromatographic efficiency was made clear and was achieved with the aid of experimental design<br/>methodology. Waters Sunfire C18 type column (150×4.6 mm, 5 μm particle size) was employed and 0.1<br/>% acetic acid in water: acetonitrile mixture was adopted as mobile phase (flow rate: 0.469 ml/min) for<br/>the separation of analytes. The developed method was validated and has been extended for the assay of<br/>marketed formulations. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS