In vitro Anti Tubercular Activity and Physicochemical Standardization of Selected Medicinal Plant Extracts
- Vol.83(2), March-April
- Mumbai Indian Journal of Pharmaceutical Science 2021
- 230-237p.
Globally tuberculosis is a major health problem. The main question with the current tuberculosis treatment is adverse e ffects and drug resistance. The present study aimed to identify safe and e ffective alternative herbal drugs for the treatment of tuberculosis. This study involves the standardization of selected medicinal plant extracts and in vitro antitubercular activity. Based on the literature survey, four medicinal plant extracts were selected i.e. Glycyrrhiza glabra (Yastimadhu), Piper longum (Pippali), Curcuma longa (Haridra) and Adhatoda vasica (Vasaka). To evaluate the authenticity and quality control aspect, chromatographic fingerprinting and physicochemical analysis were carried out. Quanti fication of phytomarkers was analyzed by High performance liquid chromatography and in vitro anti-mycobacterial activity was performed by agar well di ffusion method on Mycobacterium smegmatis . All necessary physicochemical parameters were performed and found to comply with speci fied limits. Extracts were authenticated by qualitative High-performance thin layer chromatography fingerprinting in comparison with suitable raw herbs and phytomarkers. Quanti fication by High performance liquid chromatography showed that Glycyrrhiza glabra contains 10 % Glycyrrhizin,Piper longum contains 7 % Piperine,Curcuma longa contains 82.41 % Curcumin and Adhatoda vasica contains 2.3 % Vasicine. In vitro activity of Glycyrrhiza glabra, Piper longum and Curcuma longashowed 20 mm, 16 mm and 14 mm of maximum Zone of inhibition respectively. Adhatoda vasica did not show any activity against Mycobacterium smegmatis . The present study demonstrates that the selected plant extracts have the potential to inhibit mycobacterial growth. Thus, these standardized herbal extracts could be used in the design and development of a polyherbal formulation for the treatment of primary stage tuberculosis