Shendge, Anil Khushalrao <br/><br/>
Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma 

 - Vol.53(5), Sep-Oct 
 - Mumbai  Wolter Kluwer  2021 
 - 377-383p. 
<br/><br/> INTRODUCTION: The use of natural resources as medicines for cancer therapies has been described<br/>throughout history in the form of traditional medicines. However, many resources are still unidentified<br/>for their potent biological activities.Clerodendrum viscosum is a hill glory bower reported as a remedy<br/>against oxidative stress, skin diseases, and intestinal infections.<br/>MATERIALS AND METHODS: We have collected the C. viscosum leaves and used for the<br/>preparation of 70% methanolic extract (CVLME). Then, CVLME has been confirmed for anticancer<br/>properties on various cancer cell lines by evaluating cytotoxicity, cell cycle analysis, induction of<br/>ROS and apoptosis, and nuclear fragmentation. Further, the phytochemical analysis of CVLME was<br/>evaluated through high‑performance liquid chromatography.<br/>RESULTS: Cell proliferation assay revealed the selective cytotoxicity of CVLME against breast<br/>cancer cell line (MCF‑7). The FACS‑based cell cycle analysis showed increased subG1 (apoptosis)<br/>population dose dependently. Further, the apoptosis‑inducing effect of CVLME was confirmed by<br/>annexin staining. Flow cytometry and confocal microscopy revealed the selective ROS generation<br/>upon CVLME treatment. The confocal‑based morphological study also revealed condensed and<br/>fragmented nuclear structure in CVLME‑treated MCF‑7 cells. Phytochemical investigations further<br/>indicated the presence of tannic acid, catechin, rutin, and reserpine which might be the reason for<br/>the anticancer activity of CVLME.<br/>CONCLUSION: The above‑combined results revealed the anticancer effect of CVLME, which may<br/>be due to the selective induction of ROS in breast carcinoma 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACOLOGY