Mushtaq, Z.  <br/><br/>
Isolation, identification and sequence analysis of subtilisin gene (quaking homolog) encoding a fibrinolytic enzyme from bacillus subtilis 

 - Vol.83(4), July-Aug 
 - Mumbai  Indian Journal of Pharmaceutical Science  2021 
 - 856-864p. 
<br/><br/> Fibrinolytic therapy progressed by the evolution of different strategies that helped in enhancing its efficacy<br/>and specificity. The use of microbial fibrinolytic enzymes is leading to a promising direction for the treatment<br/>of cardiovascular diseases. Subtilisin, a member of subtilases is a fibrinolytic enzyme abundantly found in<br/>Bacillus species. The isolation of subtilisin gene (quaking homolog) from Bacillus subtilis was attempted in<br/>the present work. The genomic deoxyribonucleic acid extraction was done following Yamada protocol and<br/>used as a template for polymerase chain reaction amplification of the target gene using specifically designed<br/>primers. The polymerase chain reaction product was ligated into cloning vector pTZ57R/T followed by its<br/>transformation into Escherichia coli top 10 strain. A 1090 base pair, partial gene sequence was amplified<br/>coding for subtilisin protein of 363 amino acids with molecular weight of 37550.7 Daltons. The nucleotide<br/>sequence revealed significant evolutionary relationships with subtilases from other strains of Bacillus<br/>subtilis. Our study confirms the presence of subtilisin (quaking homolog) gene in local Bacillus species<br/>suggesting economical way to produce important thrombolytic agents of commercial and pharmaceutical<br/>worth. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS