Sarkar, M.  <br/><br/>
Development and validation by reverse phase high performance liquid chromatography method for the estimation of piperine and coenzyme q10 in bulk and pharmaceutical dosage form 

 - Vol.83(6), Nov-Dec 
 - Mumbai  Indian Journal of Pharmaceutical Science  2021 
 - 1208-1214p. 
<br/><br/> Today people are focused on their health, as good health is a key to the happiness of our life. Medicine takes<br/>a big responsibility to take care of our health. Various supplements are available to meet the nutritional<br/>requirement. One such supplement is a combination of piperine and coenzyme Q10. The medicines have<br/>to be analyzed properly before introducing them to the market. As no analytical method is available for<br/>simultaneous estimation of the combination, hence an attempt was made to develop a validated method<br/>for the estimation of piperine and coenzyme Q10 simultaneously in bulk and its dosage form. A simple,<br/>precise, accurate and validated reversed phase high performance liquid chromatography technique was<br/>developed for the estimation of piperine and coenzyme Q10 in bulk and its tablet formulation. In this<br/>developed method, Waters X Bridge C 18 column (250 mm×4.6 mm, 5 μm) was used as a stationary phase<br/>and acetonitrile, tetrahydrofuran and water was used in 65:32:3 (v/v) ratio as mobile phase with 1 ml/min<br/>flow rate. This isocratic separation was accomplished using Waters 2707 Autosampler high performance<br/>liquid chromatography system, Waters 515 solvent delivery system, with photodiode array detector<br/>detection at 275 nm. Chromatographic data was processed by Empower 2 software. The retention times<br/>of coenzyme Q10 and piperine were 4.56 and 8.19 min respectively. The linearity ranges have lied between<br/>4-6 μg/ml, 240-360 μg/ml for piperine and coenzyme Q10 respectively with 0.997 as correlation coefficient<br/>for both. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS