Abdul Saleem Mohammad <br/><br/>
Determination of nitazoxanide in biological matrices by LC-MS/MS technique: method development and validation 

 - Vol.56(2), Apr-June 
 - Karnataka  Association of Pharmaceutical Teachers of India (APTI)  2022 
 - 539-545p. 
<br/><br/> Background: Determination of pharmaceuticals in the biological matrices is essential for<br/>toxicologic and pharmacokinetic applications. The main objective of the current work<br/>was to develop a bioanalytical method for quantifying nitazoxanide in biological samples<br/>by LC-MS/MS. Materials and Methods: Chromatographic elution of nitazoxanide and<br/>linagliptin were achieved on C18<br/>-hypersil (5 μ, 50x4.6mm) stationary phase with mobile<br/>phase consisting of acetonitrile and 0.1% HCOOH (75:15, v/v) processed at 0.8 ml/min<br/>flow rate. Linagliptin was utilized as an internal standard (IS). Results: The method<br/>was linear in the concentration levels of 0.53–21.2 ng/ml with more than 0.999<br/>r2<br/>values, consisting of 0.53 ng/ml as the lower limit of quantification (LLOQ). Analytes<br/>were subjected to pretreatment by liquid-liquid extraction (LLE) procedure with average<br/>extraction recovery findings within 99.93±5.04%. Method accuracy findings were<br/>present in between 96.44% to 104.31%, and the assessed percentage relative standard<br/>deviation findings for within and between run precision were ≤ 4.68%. Conclusion: The<br/>developed LC-MS/MS procedure for quantitating nitazoxanide in the biological matrix<br/>was suitable for routine analysis of patients’ blood samples for pharmacokinetic studies<br/>and drug monitoring. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS