Mechanism of long non-coding RNA MAGI2- antisense RNA 3 targeting microRNA-31-5p to regulate proliferation and metastasis of SiHa cells in cervical cancer
- Vol.84(2), Mar-Apr
- Mumbai Indian Journal of Pharmaceutical Science 2022
- 477-482p.
To investigate the effect of long non coding RNA MAGI2-antisense RNA 3 on the proliferation, invasion and migration of cervical cancer cells and the underlying mechanism. SiHa cells were divided into plasmid cloning DNA group, plasmid cloning DNA-MAGI2-antisense RNA 3 group, anti-microRNA-negative control group, anti-microRNA-31-5p group, plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA- negative control group and plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA-31-5p group; quantitative reverse transcription polymerase chain reaction was used to determine the messenger RNA expression levels of microRNA-31-5p and MAGI2-antisense RNA 3; cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay; the number of cell migration and invasion in each group was detected by Transwell assay; dual luciferase reporter assay was performed to detect the fluorescence activity. The expression levels of microRNA-31-5p were significantly increased and those of MAGI2-antisense RNA 3 messenger RNA were decreased in cervical cancer cell lines (p<0.05); overexpression of MAGI2-antisense RNA 3 or inhibition expression of microRNA-31-5p could suppress SiHa cell proliferation, migration and invasion (p<0.05). MAGI2-antisense RNA 3 targeted regulation of microRNA-31-5p; overexpression of microRNA-31-5p partially reversed the inhibitory effects of overexpressing MAGI2-antisense RNA 3 on the proliferation, migration, invasion of SiHa cells. Long non coding RNA MAGI2-antisense RNA 3 can inhibit cervical cancer cell proliferation, migration and invasion by regulating microRNA-31-5p.