Jeong, S. M.  <br/><br/>
Anti-inflammatory effects of sargassum muticum ethanol extract in lipopolysaccharide-induced raw 264.7 cells and mouse ear edema models 

 - Vol.84(1), Jan-Feb 
 - Mumbai  Indian Journal of Pharmaceutical Science  2022 
 - 230-238p. 
<br/><br/> This study investigated the effects and the anti-inflammatory activity of Sargassum muticum ethanol<br/>extract in lipopolysaccharide-induced RAW 264.7 murine macrophage cells and in a croton oil-induced<br/>mouse ear edema model. Pretreatment of lipopolysaccharide-induced RAW 264.7 cells with Sargassum<br/>muticum ethanol extract (0.1-100 μg/ml) inhibited lipopolysaccharide-induced production of nitric<br/>oxide, interleukin-6 and tumour necrosis factor alpha in a dose-dependent manner. The expression of<br/>lipopolysaccharide-induced, inducible nitric oxide synthase and cyclooxygenase-2 in the Sargassum<br/>muticum ethanol extract-treated group was also suppressed in a dose-dependent manner. Furthermore, we<br/>found that Sargassum muticum ethanol extract induced anti-inflammatory effects by inhibiting Mitogen-<br/>activated protein kinases (extracellular signal-regulated kinase, c-Jun N-terminal kinase and p38) and<br/>nuclear factor kappa B, p65 phosphorylation in lipopolysaccharide-stimulated RAW 264.7 cells. The anti-<br/>inflammatory activity of Sargassum muticum ethanol extract in vivo was evaluated in the ear edema model<br/>of a croton oil-treated mouse. Compared to the untreated control, croton oil-induced ear edema was found<br/>to be reduced by about 33 % upon treatment with 250 mg/kg Sargassum muticum ethanol extract. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS