Bioanalytical method for estimation of teriflunomide in human plasma
- Vol.14(9)
- M P Innovare Academic Sciences Pvt Ltd 2022
- 19-23p.
Objective: Teriflunomide is used for the treatment of multiple sclerosis and is available in 7 mg and 14 mg tablets. This study aimed to develop and validate a simple and economical HPTLC method for the estimation of Teriflunomide in human plasma.Methods: HPTLC method was developed using toluene: ethyl acetate: acetic acid as the mobile phase and the stationary phase was a TLC plate precoated with silica gel 60 F254. The detection wavelength set was 294 nm. The sample preparation involved a simple protein precipitation technique with Acetonitrile as a precipitating protein agent; the internal standard selected was Rilpivirine. The validation was carried out as per bio-analytical method guidelines.Results: The Rf value for Teriflunomide was found to be 0.46±0.04. The linearity range was observed from 10-60 μg/ml with a regression coefficient value of 0.9819. The developed method was validated for various parameters like specificity, linearity, accuracy, precision, recovery, and stability.Conclusion: The developed method is simple, specific, accurate, and economical for the estimation of Teriflunomide in human plasma.