Wang, Y.  <br/><br/>
Identification of the absorbed components of raw fuzi (lateral root of aconitum carmichaelii debx.) in a rat adjuvant arthritis model 

 - Vol.84(5), Sep-Oct 
 - Mumbai  Indian Journal of Pharmaceutical Science  2022 
 - 1241-1247p. 
<br/><br/> The aim of the present study was to identify the absorbed components of raw fuzi in a rat adjuvant arthritis<br/>model and evaluate the therapeutic effects of three alkaloids of fuzi’s absorbed components, aconitine,<br/>hypaconitine and mesaconitine. The adjuvant arthritis model was established by complete Freund’s<br/>adjuvant injection in Wistar rats. Then the animal’s body weight, condition of fur, hind paw volume and<br/>immunological parameters, nitric oxide and tumor necrosis factor-alpha, were assessed as markers of<br/>inflammation and arthritis. Serum samples from rats treated with oral fuzi extracts were analyzed by<br/>ultra performance liquid chromatography-mass spectrometric and 12 prototypes of fuzi were identified.<br/>Aconitine and mesaconitine could substantially reduce the serum levels of nitric oxide and tumor necrosis<br/>factor-alpha in the adjuvant arthritis model, while hypaconitine did not show obvious effects. The method<br/>for the identification of absorbed components of raw fuzi was simple and sensitive. Among the alkaloids,<br/>aconitine and mesaconitine might be the major compounds from diester-diterpenoid alkaloids for the<br/>treatment of rheumatoid arthritis. The current study connected serum pharmacochemistry study of fuzi<br/>with pharmacological experiment in adjuvant arthritis model and discovered the effective substance of<br/>fuzi, which could provide plausible evidence for its using, a candidate treatment for rheumatoid arthritis. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS