Mahdi, Abbas Ali  <br/><br/>
Metabolite profiling and antioxidant activity of leaves, stem, and flowers of Tridax procumbens  : A medicinal herb 

 - Vol.17(1), Jan-Mar 
 - Mandsaur  BRNSS Publication Hub.  2023 
 - 76-84p. 
<br/><br/> Introduction: This study was designed to analyze in vitro antioxidant activity and estimate the metabolic profiling of<br/>non-polar and polar extracts of Tridax procumbens leaves stem and flowers. Materials and Methods: Antioxidant<br/>activity of hexane and methanol extract was determined through DPPH free-radical scavenging assay and nitric<br/>oxide assay using the standard method and metabolite profiling of non-polar and polar extracts of T. procumbens<br/>was done by using gas chromatography-Mass spectrometry (GC-MS). Results: The hexane extract of<br/>T. procumbens flower (HTPF) showed the most significant antioxidant activity concerning the methanolic extract,<br/>which is indicated by its IC50 values obtained from the DPPH was 33.14 μg/mL and 34.52 μg/mL (HTPF and<br/>MTPL) wherein IC50 for nitric oxide assay was 16.74 and 34.26 μg/mL (HTPF and MTPL), respectively. In<br/>addition, GC-MS-based metabolic profiling of T. procumbens parts (leaves, stem, and flowers) showed hundred<br/>and more chemically diverse metabolites. A very high concentration of oleanolic acid was found in hexane leaves<br/>(32.13 ± 0.3.11%) and flowers (17.66 ± 1.71%) extract while in stems (12.63 ± 1.22). The other metabolites<br/>such as 2, 4-di-tert-butyl phenol, and germacrene were identified as major metabolites. Trans-farnesol and<br/>two major fatty acids palmitic and linolenic and high percent peak area of stearic acid were detected in stems.<br/>Discussion and Conclusion: GC-MS analysis showed several compounds with, high content of terpenes and<br/>sterols which may be a reason for their antioxidant activity. The antioxidant property of medicinal plants has a<br/>different role in combating various diseases. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS