Lay, Sammy Henry  <br/><br/>
Viability of human dental pulp stem cells: The potential of L‑arginine‑based culture media 

 - Vol.14(4), Oct-Dec 
 - Mumbai  Wolter Kluwer  2023 
 - 306-310p. 
<br/><br/> Dental pulp is built by proteins that have various roles in the biological process of<br/>pulp, such as structural protein, regulation protein, and catalytic protein. L‑arginine,<br/>an amino acid and one of the building blocks of proteins, regulates pro‑inflammatory<br/>and anti‑inflammatory activity. Therefore, L‑arginine‑based culture has potential<br/>to promote dental pulp regeneration. This study aimed to investigate the potential<br/>of L‑arginine‑based culture in improving the viability of human dental pulp stem<br/>cells (hDPSCs). We evaluated the viability of hDPSCs in culture media supplemented<br/>with different concentrations of L‑arginine amino acid (250, 300, 350, and 400 μmol/L)<br/>and Dulbecco’s Modified Eagle Medium plus fetal bovine serum 10% (control)<br/>using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay after<br/>24‑h incubation time. Statistical analysis was conducted using a one‑way analysis of<br/>variance and post hoc least significant difference test. In qualitative analysis, the 4´,<br/>6‑diamidino‑2‑phenylindole staining method was used. The evaluation has shown a<br/>significant result when 250, 300, and 350 μmol/L concentration of L‑arginine amino acid<br/>culture media compared with control, and 400 μmol/L has the best result and was not<br/>significantly different with control toward viability of hDPSCs. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS