TGF-ò/Smad/ADAMTS-7 Axis regulates the process of curcumin in promoting the cartilage cells proliferation
- Vol.85(3), May-Jun
- Mumbai Indian Journal of Pharmaceutical Science 2023
- 835-840p.
To explore the function of curcumin on the cartilage cells, the cell proliferation and apoptosis by transforming growth factor-beta/suppressor of mothers against decapentaplegic/a disintegrin and metalloproteinase with thrombospondin motifs-7 axis was the objective of the study. The cell viability was detected by cell counting kit-8. The expression of transforming growth factor-beta, suppressor of mothers against decapentaplegic protein, a disintegrin and metalloproteinase with thrombospondin motifs-7, caspase-9, B-cell lymphoma 2, Bcl-2-associated X protein was determined by Western blot. The cartilage cells were treated with 1 mmol/l sodium nitroprusside for 24 h. Then, the cells were treated with different concentration of curcumin for 24 h. We found that 1 μmol/l curcumin could recover the cartilage cells proliferation. The transforming growth factor-beta and suppressor of mothers against decapentaplegic protein show high expression and a disintegrin and metalloproteinase with thrombospondin motifs-7 was low in the curcumin treatment group. Meanwhile, the apoptosis pathway was also detected. The caspase-9 and B-cell lymphoma 2 was higher in the curcumin treatment group than without curcumin group. However, the Bcl-2-associated X protein was lower. The cell viability, a disintegrin and metalloproteinase with thrombospondin motifs-7, caspase-9, B-cell lymphoma 2 and Bcl-2-associated X proteins also show no changes with or without curcumin when the transforming growth factor-beta was inhibited. We found that a disintegrin and metalloproteinase with thrombospondin motifs-7 show over expression, the transforming growth factor-beta and suppressor of mothers against decapentaplegic protein show high expression in the curcumin treatment group, the cell viability, caspase-9, B-cell lymphoma 2 and Bcl-2-associated X proteins show no changes with or without curcumin. Curcumin can promote the cartilage cells proliferation via transforming growth factor-beta/suppressor of mothers against decapentaplegic/a disintegrin and metalloproteinase with thrombospondin motifs-7 axis and inhibit the cell apoptosis.