Nesarikar, Mugdha  <br/><br/>
Modulation of the activity of human plasma esterases by binary and ternary mixtures of water miscible organic solvents during in vitro drug metabolism studies 

 - Vol.85(4), Jul-Aug 
 - Mumbai  Indian Journal of Pharmaceutical Science  2023 
 - 1157-1162p. 
<br/><br/> Organic solvents are commonly used in in vitro drug metabolism studies as co-solvents for lipophilic substrate/<br/>new chemical entities. The effects of 1 % v/v final concentration of individual, binary and ternary mixtures of<br/>methanol, acetonitrile and dimethyl sulfoxide on human plasma esterases were investigated. The hydrolysis<br/>of p-nitrophenyl acetate to p-nitrophenol was monitored by ultraviolet spectrophotometry to follow<br/>esterase activity. Results indicated that the mixtures always showed less inhibition of esterase as compared<br/>to theoretically calculated additive inhibition based on individual solvent data. Results also indicated that<br/>dimethyl sulfoxide was the most-inhibitory solvent (59-64 % inhibition at 1 % v/v final concentration in<br/>incubation) and mixtures containing the least concentration of dimethyl sulfoxide caused the least inhibition<br/>of human plasma esterases. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS