Pharmacokinetic study of zandopaŽ in rat model
- Vol.85(4), Jul-Aug
- Mumbai Indian Journal of Pharmaceutical Science 2023
- 1163-1168p.
The goal of this research is to examine the pharmacokinetics of ZandopaŽ in rats after oral administration of ZandopaŽ herbal formulation. Acclimatization of rats was done for 7 d before the study. The study consisted of 24 rats which had been separated equally into two groups i.e. vehicle control and ZandopaŽ control group with 12 animals in each group. The drug was administered via oral route in male Sprague Dawley rats. The vehicle control group rats were dosed with 1 % carboxymethyl cellulose and ZandopaŽ control group with 775 mg/kg of ZandopaŽ in 1 % carboxymethyl cellulose. Food was with-held for a further 1 h. The blood sample was taken between 0, 0.5, 1, 2, 4, 6, 8, 12 and 24 h time intervals after treatment with the respective drug. Plasma samples were prepared by centrifugation and concentration was measured by high performance liquid chromatography. Urine was collected at a time interval of 0-1, 1-4, 4-8, 8-24, 24-48 h after treatment with the respective drug, and kinetic parameters were measured. Then animals were sacrificed using isoflurane overdose and the liver was isolated and stored for metabolism study. The Cytochrome P-450 concentration and Cytochrome P-450 content was found to be 0.0012 mM and 1.2 nmol/mg/protein compared with the vehicle control group. The cumulative amount excreted was found to be 64.66 mg. The study of the pharmacokinetics of ZandopaŽ in rats was shown to be significant as compared to the vehicle control group.