Phytochemical profiling using LC-Q-TOF-MS analysis and in vitro antioxidant activtiy of a rere salt-secreting mangrove aegialitis rotundifolia roxb leaves extract
- Vol. 2(3)
- M P Innovare Academic Sciences Pvt Ltd 2019
- 37-47p.
Objective: The present work deals with the qualitative study of the phytoconstituents present in Aegialitis rotundifolia Roxb., ethanolic leaves extract and evaluate its antioxidant properties in vitro . Metho ds: The qualitative phytochemical analysis of the extract was performed first using preliminary phytochemical tests and then by liquid chromatography quadrupole -time -of -flight mass spectrometry (LC -Q-TOF -MS ). The antioxidant properties were investigated co mprehensively using seven in vitro models viz., 2, 2 -diphenyl -1-picrylhydrazyl ( DPPH ) radical scavenging, n itric oxide (NO) scavenging, hydrogen peroxide (H 2 O 2 Result s: The preliminary phytochemical analysis revealed the presence of several important phytochemical groups whereas the LC -Q-TOF -MS analysis detected 2 5 phytoconstituents in the extract mostly belonging to flavon oids and alkaloids . The test extract showed strong dose -dependent antioxidant activity in all the seven in vitro models, however , the activity of the extracts was slightly lower compared to the reference standard ascorbic acid. ) scavenging, superoxide (SOD) radical scavenging, l ipid peroxidation (LPO) assay, reducing power (RP), and t otal antioxidant activity. Conclusio n: The test extrac t showed strong antioxidant properties which could be possibly due to the phytoconst ituents detected in the extract.