Marwa, AlJamal K. <br/><br/>
DEVELOPMENT AND VALIDATION OF ANALYTICAL SPECTROPHO TOMETRIC AND RP-HPLC  METHODS FOR THE SIMULTANEOUS ESTIMATION OF HYDROQUI NONE, HYDROCORTISONE AND  TRETINOIN TERNARY MIXTURE IN TOPICAL FORMULATION  

 - Vol.11(11) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 10-16p. 
<br/><br/> Objective: <br/>Development and validation of spectrophotometric an<br/>d RP-HPLC methods for the simultaneous determination<br/> of Hydroquinone (HQ), <br/>Hydrocortisone (HC) and Tretinoin (TRT) ternary combi<br/>nation in pharmaceutical preparation. <br/>Methods: <br/>The proposed spectrophotometric method was able to <br/>determine TRT directly from its absorption spectrum<br/> at 362 nm, however, HQ and <br/>HC from their first derivative spectra at 284 nm and<br/> 252 nm, respectively, without any separation step.<br/> The RP-HPLC method was developed using a <br/>C<br/>18 <br/>Sunfire<br/>©<br/> waters column with a mobile phase composed of acet<br/>onitrile: phosphate buffer (adjusted to pH 6.1 using<br/> ortho-phosphoric acid) in the <br/>ratio of 30:70 %, v/v, respectively at a flow rate <br/>of 0.8 ml/min. Quantification was based on measurin<br/>g peak areas at 260 nm. <br/>Results: <br/>The spectrophotometric method was able to selective<br/>ly quantify each of HQ, HC and TRT in the ranges of<br/> 10-50 μg/ml, 2-10 μg/ml and 0.5-5 μg/ml, <br/>respectively. The RP-HPLC method was able to produc<br/>e well-resolved peaks after 3.0, 8.2 and 20.2 min, <br/>in the ranges of 2-10 μg/ml, 0.1-1 μg/ml and 0.05-2<br/>μg/ml, for HQ, HC and TRT, respectively. The obtain<br/>ed A, D<br/>1<br/> or peak areas values plotted against the concentra<br/>tion of each of the three components showed <br/>linear response in the stated ranges. Both methods <br/>were validated in terms of linearity, LOD, LOQ, pre<br/>cision, accuracy and selectivity. <br/>Conclusion: <br/>Both developed proposed methods were applied for th<br/>e determination of the active ingredients in the ph<br/>armaceutical formulation and <br/>the  common  excipients  did  not  interfere  in  the  anal<br/>ysis.  The  RP-HPLC  method  proved  to  be  more  sensitiv<br/>e  when  compared  to  the  applied <br/>spectrophotometric method. However, the applied spe<br/>ctrophotometric methods, considered as green analyt<br/>ical chemistry, is a simple, time-saving <br/>method that requires minimal use of a hazardous sol<br/>vent.   
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS