POLYPHENOL CONTENT, PHYTOCHEMISTRY COMPOSITIONS AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS FROM MARINE SPONGE AAPTOS SUBERITOIDES GROWN IN NHATRANG BAY, VIETNAM
- Vol.11(9)
- M P Innovare Academic Sciences Pvt Ltd 2019
- 80-86p.
Objective: To investigate the content and antioxidant activiti es of polyphenol, the correlation between polypheno l content and their antioxidant activities, and phytochemistry compositions of diff erent extracts from marine sponge Aaptos suberitoides commonly found growing in Nhatrang bay, Vietnam orienting application into functional food and pharmacy. Methods: Evaluating the toxicity of antioxidant polyphenol powder preparing from the initial concentrated extr act was by the adjusted Behrens Karber method and a correlation between polyphenol content and antioxidant activities basing on the Pe arson coefficient in Excel. Separating antioxidant polyphenol content was base on solvents polarization of n-hexane, chloroform, ethanol, eth yl acetate and n-butanol which the quantification of polyphenol content and antioxidan t activities, and preliminary phytochemical composi tions qualitative. Results: Antioxidant polyphenol powder did not affect mice w eight during the assay time of 28 d. Polyphenol con tent and antioxidant activities got the highest value at chloroform extract in comparis on to other extracts, a significant difference (p<0 .05) and strong correlation (R2>0.9). Polyphenol content (122.682 mg gallic acid equivale nt ml-1), total antioxidant activity (368.183 mg as corbic acid equivalent ml-1), reducing power activity (24.08 mg FeSO4 equivalent ml-1) and DPPH s cavenging (72.48±1.54 %) were the highest values. A lkaloids, flavonoids, steroids, tannins and triterpenoids existed in initial methanol extra ct. Weakly polarized polyphenol content was 70.27% in comparison to initial methanol extract.