Dermane, Affo <br/><br/>
ANTISICKLING ACTIVITY EVALUATION OF FRACTIONS OBTAINED FROM WHOLE EXTRACTS  OF  NEWBOULDIA  LAEVIS  P. BEAUV (BIGNONIACEAE )  

 - Vol.11(2) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 42-46p. 
<br/><br/> Objective: <br/>This study aims to <br/>evaluate the fractions from whole extracts of roots and stem barks of <br/>Newbouldia <br/>laevis<br/>and their effect on sickling<br/>. <br/>Method<br/>s: <br/>Hydroethanolic <br/>extracts <br/>of stem bar<br/>ks and  root<br/>s of <br/>Newbouldia<br/> laevis<br/>we<br/>re fractionated by <br/>the technics of cold precipitation in ethanol. <br/>The  fractions  obtained  after  phytochemical<br/>  screening  were  subjected  to<br/>  Emmel  test  to  evaluate  their  anti<br/>-sickling  activity.  Active  fractions  were  <br/>tested for DPPH and AAPH assay<br/> (AAPH induced m<br/>embrane<br/> lipoperox<br/>idation<br/> and evaluation of <br/>reduction of <br/>hemolysis)<br/>. <br/>Result<br/>s: <br/>Two fractions were obtained from each whole extrac<br/>t:    supernatant and pellet fractions.<br/> Supernatants fractions obtained from whole roots <br/>bark<br/>s extract and stem barks extract <br/>at a concentration of 3<br/>0 mg<br/>/ml<br/> reduced sickling up respectively to 7<br/>% and <br/>10%<br/> against 86% for the control<br/>. <br/>Pellets fractions obtained from <br/>the both<br/> extracts induced coagulation of SS blood<br/> at 30 mg<br/>/ml<br/> against 86% for the control<br/>. <br/>Conclusio<br/>n: <br/>Supernatants fractions of hydroethano<br/>lic whole extract of roots and stem backs of <br/>Newbouldia <br/>laevis<br/> promise as the potential source of <br/>active molecules against sickle cell disease.   
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS