Puri, Smita K. <br/><br/>
CHARACTERIZATION , IN VITRO  ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF  FUNGAL ENDOPHYTIC EXTRACTS OF  ANDROGRAPHIS PANICULATA  LEAVES IN CCL Original Article 4  INDUCED  HEPATOTOXICITY 

 - Vol.11(1) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 44-54p. 
<br/><br/> Objective: <br/>To isolate and characterize the endophytic fungi from the leaves of<br/>Andrographis paniculata <br/>for free radical scavenging antioxidant <br/>and <br/>hepatopr<br/>otective activity against <br/>CCl<br/>4<br/>Method<br/>s: <br/>Two  fungal  <br/>endophytes, <br/>APLF<br/>-3 (<br/>Andrographis <br/>paniculata<br/>  leaf  fungi<br/>-3)  and  APLF<br/>-4 (<br/>Andrographis <br/>paniculata<br/>  leaf  fungi<br/>-4) <br/>were isolated <br/>from<br/>  leaves  of  <br/>Andrographis <br/>paniculata<br/>to  get  chloroform  (A3C,  A4C),  ethyl  acetate  (A3EA,  A4EA)  and  n  butanol  (A3nB,  A4nB)  extracts.  rDNA <br/>sequencing by PCR technique was carried out for identification of APLF<br/>-3 and APLF<br/>-4. All the APLF<br/>-3 and APLF<br/>-4 extracts were assayed for <br/>in vitro<br/>free  radical  scavenging  activity  against  2,  2<br/>-diphenyl<br/>-1-picrylhydrazyl  (DPPH),  hydroxyl  radical  and  redu<br/>cing  power.  Then,  A4EA  and  A4nB  were  <br/>screened for hepatoprotective activity against CCl<br/> induced hepatotoxicity<br/>4<br/>Result<br/>s: <br/>The endophytic fungi, APLF<br/>-3 and <br/>APLF<br/>-4, were identified as <br/>Phyllosticta sp. ZLY<br/>-2010 isolate M13 <br/>and <br/>Aspergillus <br/>tubingensis<br/> strain Cs/7/2 <br/>respectively  based  on  their  morphological  and  molecular  characterization.  A4EA  and  A4nB  showed  significant  <br/>in  vitro<br/>  free  radical  scavenging  <br/>activity  as  compared  to  other  extracts. <br/>A4EA  and  A4nB  (50  mg/kg  <br/>and<br/>100  mg/kg)  reversed  the  increased  serum  biochemical  parameters  as  <br/>compared to CCl<br/> induced hepatotoxicity at 50 mg/kg and<br/>100 mg/kg doses.<br/>4<br/>Conclusio<br/>n: <br/>These   findings   suggested   that   the   extracts   (A4EA   and   A4nB)   obtained   from   <br/>endophytic   fungi   APLF<br/>-4   contributed   towards   <br/>hepatoprotective activity. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS