MOLECULAR DOCKING STUDIES AND SYNTHESIS OF 3, 4 - DISUBSTITUTED TRIAZOLES AS Original Article MYCOBACTERIUM TUBERCULOSIS ENOYL -ACP REDUCTASE AND CYP -51 INHIBITORS
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Objective: To design, synthesize and in vitro antitubercular , antifungal and antioxidant evaluation of some novel mercapto 1, 2, 4–triazole derivatives. Method s: New derivatives were designed by using various software like ACD Lab chemsketch , mol inspiration and autodock . Designed molecules are obeying Lipinski’s rule of five and having highest binding score w as selected for the synthesis. The synthesized compounds were subjected to TLC, melting point determination, FTIR, 1 H NMR, 13 Result s: A virtual screening was carried out through docking designed compounds into the I nhA and CYP -51 binding site to predict if these compounds have an analogous binding mode o f the C NMR and mass spectral analysis. The newly synthesized compounds were investigated for in vitro antitubercular evaluation by MABA me thod, antifu ngal evaluation by cup plate method and antioxidant evaluation by DPPH scavenging assay. enoyl ACP reductase (InhA) and CYP -51 inhibitors. Three der ivatives (4a1, 4a2 and 4a3 ) were selected for the synthesis with the help of in silico modeling . The selected derivatives were synthesized by a conventional method. All the synthesized compounds showed a characteristic peak in FT IR, 1 H and 13 Conclusio n: The derivatives were synthesized adopting simple and laboratory friendly reaction conditions to give the target compounds in quantitative yields. Newer derivatives possess good antitubercular, antifungal and antioxidant activity.