Karthik, S. <br/><br/>
Analytical method development and its validation for evaluation of polyherbal formulation 

 - Vol. 55 (10) 
 - Mumbai  Indian Drug Manufacture's Association - IDMA  2018 
 - 66-68 
<br/><br/> Talekt capsule is a branded polyherbal formulation used for enhancing the immune response to dermal infections and also for treating skin disorders. Curcumin and embelin, which are the active constituents of Haridra and Vidanga, respectively were used as marker compounds for developing a simple, accurate, precise and robust HPTLC method for simultaneous estimation. The mobile phase of toluene: ethyl acetate: formic acid (6.5: 3: 0.2 v/v/v) was used for separation. 381nm was used as wavelength for analysis. The Rf value obtained for curcumin, and embelin was found to be 0.51 ± 0.2 and 0.33 ± 0.2, respectively. The developed method was validated on the basis of ICH Q2 (R1) guidelines. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS
<br/><br/><label>Subjects--Index Terms: </label>Curcumin  Embetin  Simultaneous estimation  HPTLC