Chibi, Fatima <br/><br/>
Screening of the antioxidant activity of crude extracts in 86 algae species from el jadida coast (Morocco) 

 - Vol. 2(3) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 54-61p. 
<br/><br/> Objective: <br/>This <br/>work <br/>aimed to <br/>screen <br/>the antioxidant activity of marine macroalgae from the Moroccan Atlantic coast (region of El Jadida).<br/>Method<br/>s: <br/>Evaluation    of    the    antioxidant    activity    of    different    collected    species,    lyophilized    and    extracted    with    a    solvent    mixture    <br/>chloroform<br/>/methanol<br/> (2/1; v<br/>/v) <br/>was <br/>conducted according to two techniques, first by thin layer chromatography (<br/>tlc<br/>) then by spectrophotometry, <br/>using  a  free  radical  2,2<br/>-diphenyl<br/>-1-picrylhydrazyl  (<br/>dpph<br/>).  The  sampling  on  a  distance  of  110  km  allowed  to  harvest  86  algal  specie<br/>s  (16  brown  <br/>algae, 47 red algae, 14 green algae and 9 algae <br/>being identified). <br/>Result<br/>s: <br/>The analys<br/>is by thin layer chromatography reveals an antioxidant activity in nearly half of harvested algal species (52.32 %). This act<br/>ivity <br/>varies depending on the concentration of the extract and in function of incubation time in the presence of <br/>dpph<br/>. The monitori<br/>ng  of  the  <br/>kinetic<br/>s of   <br/>degradation of <br/>dpph<br/> by spectrophotometer in the presence of extracts which were active by <br/>tlc<br/> allowed to confirm the results and select the most <br/>active  algal  species  based  on  the  percentage  of  remaining  dpph<br/>  in  the  medium  after  120  <br/>min  of  reactio<br/>n: <br/>Fucus <br/>spiralis<br/>(17.02  %)<br/>,  Cyctoseira<br/>ericoides<br/>(12.16 %)<br/>(Ph<br/>aeophyceae)<br/>,<br/>and<br/> Gracilaria<br/> multipartita<br/>(36%)<br/>, Halopitys <br/>incurvus<br/>(5%) (Rhodophyceae).  <br/>Conclusio<br/>n: <br/>Keyword<br/>s: <br/>Marine algae<br/>, Morocco<br/>, Extracts<br/>, Antioxidant <br/>activity,<br/> Dpph<br/>The results show that the methodology adopted in this work is reliable and can be used for rapid screening of antioxidant pro<br/>perty in <br/>plants  and  the  species<br/>: <br/>Fucus <br/>spiralis<br/>,  Cyctoseira<br/>  ericoides<br/>,  Gracilaria<br/>  multipartita<br/>, <br/>and<br/>Halopitys <br/>incurvus<br/>can  be  a  promising  source  of  natural  <br/>compounds endowed with high<br/> antioxidant potential. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS