Anlysis of fatty acids from oil of green tea (camellia sinensil l) by gas chromatography coupled with flame ionization detector and its anticancer and antibacterial activity in vitro
- Vol. 2(3)
- M P Innovare Academic Sciences Pvt Ltd 2019
- 104-111p.
Objective: Tea is a widely consumed beverage worldwide . The effec t of green tea is mainly due to its high polyphenols -(-) epigallocatechin -3-gallate (EGCG) content in the culture of cancer cell and bacterial cells . The present work was carried out to investigate the efficacy of green tea oil ( GTO) against cancer cells and bacterial cells . Method s: In this study green tea oil was prepared from green tea for different experiment and determination of fatty acids profile fro m green tea oil. In the present study, peripheral blood lymphocyte ( PBL ) was chosen as human peripher al blood lymphocytes and blood cancer MCF -7 cells were chosen as human cancer cells. To fulfill our aims and also to evaluate the activity of this phytomedicine against normal lymp hocytes and cancer cells the cell samples were divided into 26 experimental groups in the following ways. Each Petri dish contains 2 X 10 5 Result s: GTO shows a potent anticancer agent but nontoxic to normal cells. The GTO decrease s the reduced glutathione ( GSH ) level and increase the oxidized glutathione (GSSG ) level signif icantly (P <0.05) in MCF -7 cells. But in lymphocytes the GSH level and GSSG level were almost the same with the control group but doxorubicin (DOX ) significantly decreased the GSH and increase the GSSG level. Green tea oil treatment causes generation of rea ctive oxygen species (ROS ) in MCF -7 cells revealed by DCFH cells. 2 Conclusio n: This phytomedicine ha s a potent anticancer activity without damaging the normal lymph ocytes. So, this drug can be used for further treatment of anticancer and antibacterial .