Evaluation of Real Time Cell Proliferation, Anti- Inflammatory and Wound Healing Potential of Helenalin on HaCaT Keratinocytes Treated with Lipopolysaccharide Stimulated Monocytes
By: YUKSEL, S. N
.
Contributor(s): DIKMEN, M.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(2), March-April.Description: 219-229p.Subject(s): PHARMACEUTICSOnline resources: Click here
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Indian journal of pharmaceutical sciencesSummary: Arnica montana, of the family Asteraceae, is used for its anti-inflammatory and wound healing properties,
especially for bruises, tissue injuries and other traumas. However, its cellular and molecular mechanisms
are not yet fully known. Its main active ingredient is helenalin, a sesquiterpene lactone, known as one of
the main active ingredients of Asteraceae species. The aim of this study was to investigate cell proliferation,
wound healing and anti-in flammatory activity of helenalin on human keratinocyte cells treated with
lipopolysaccharide stimulated human monocytic cell line. Human keratinocyte cells were treated with
different helenalin concentrations (0.095-50μM) to determine toxic and nontoxic concentrations. Then, the
effects of nontoxic (0.02 and 0.2μM) and toxic (2 μM) helenalin concentrations on human keratinocyte cell
proliferation and migration were determined using the real time cell analysis system. The scratch assay was
performed to determine wound healing activity and supernatants were collected to determine the e ffects
of helenalin on cytokine levels of human keratinocyte cells under in flammatory and non-in flammatory
conditions. Under inflammatory conditions, 0.02 and 0.2μM helenalin increased keratinocyte proliferation
and induced the most wound healing and anti-in flammatory activity in human keratinocyte cells. Cell
proliferation and migration, wound healing and anti-in flammatory effects were higher in cells exposed
to 0.02 μM helenalin than 0.2 μM. Helenalin has been found to decrease the production of in flammatory
cytokines, especially under in flammatory conditions and increase wound closure by increasing cell
proliferation and migration.
Arnica montana, of the family Asteraceae, is used for its anti-inflammatory and wound healing properties,
especially for bruises, tissue injuries and other traumas. However, its cellular and molecular mechanisms
are not yet fully known. Its main active ingredient is helenalin, a sesquiterpene lactone, known as one of
the main active ingredients of Asteraceae species. The aim of this study was to investigate cell proliferation,
wound healing and anti-in flammatory activity of helenalin on human keratinocyte cells treated with
lipopolysaccharide stimulated human monocytic cell line. Human keratinocyte cells were treated with
different helenalin concentrations (0.095-50μM) to determine toxic and nontoxic concentrations. Then, the
effects of nontoxic (0.02 and 0.2μM) and toxic (2 μM) helenalin concentrations on human keratinocyte cell
proliferation and migration were determined using the real time cell analysis system. The scratch assay was
performed to determine wound healing activity and supernatants were collected to determine the e ffects
of helenalin on cytokine levels of human keratinocyte cells under in flammatory and non-in flammatory
conditions. Under inflammatory conditions, 0.02 and 0.2μM helenalin increased keratinocyte proliferation
and induced the most wound healing and anti-in flammatory activity in human keratinocyte cells. Cell
proliferation and migration, wound healing and anti-in flammatory effects were higher in cells exposed
to 0.02 μM helenalin than 0.2 μM. Helenalin has been found to decrease the production of in flammatory
cytokines, especially under in flammatory conditions and increase wound closure by increasing cell
proliferation and migration.
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