Viability of human dental pulp stem cells: The potential of L‑arginine‑based culture media
By: Lay, Sammy Henry
.
Contributor(s): Margono, Anggraini.
Publisher: Mumbai Wolter Kluwer 2023Edition: Vol.14(4), Oct-Dec.Description: 306-310p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Journal of advanced pharmaceutical technology and researchSummary: Dental pulp is built by proteins that have various roles in the biological process of
pulp, such as structural protein, regulation protein, and catalytic protein. L‑arginine,
an amino acid and one of the building blocks of proteins, regulates pro‑inflammatory
and anti‑inflammatory activity. Therefore, L‑arginine‑based culture has potential
to promote dental pulp regeneration. This study aimed to investigate the potential
of L‑arginine‑based culture in improving the viability of human dental pulp stem
cells (hDPSCs). We evaluated the viability of hDPSCs in culture media supplemented
with different concentrations of L‑arginine amino acid (250, 300, 350, and 400 μmol/L)
and Dulbecco’s Modified Eagle Medium plus fetal bovine serum 10% (control)
using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay after
24‑h incubation time. Statistical analysis was conducted using a one‑way analysis of
variance and post hoc least significant difference test. In qualitative analysis, the 4´,
6‑diamidino‑2‑phenylindole staining method was used. The evaluation has shown a
significant result when 250, 300, and 350 μmol/L concentration of L‑arginine amino acid
culture media compared with control, and 400 μmol/L has the best result and was not
significantly different with control toward viability of hDPSCs.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2024-0089 |
Dental pulp is built by proteins that have various roles in the biological process of
pulp, such as structural protein, regulation protein, and catalytic protein. L‑arginine,
an amino acid and one of the building blocks of proteins, regulates pro‑inflammatory
and anti‑inflammatory activity. Therefore, L‑arginine‑based culture has potential
to promote dental pulp regeneration. This study aimed to investigate the potential
of L‑arginine‑based culture in improving the viability of human dental pulp stem
cells (hDPSCs). We evaluated the viability of hDPSCs in culture media supplemented
with different concentrations of L‑arginine amino acid (250, 300, 350, and 400 μmol/L)
and Dulbecco’s Modified Eagle Medium plus fetal bovine serum 10% (control)
using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay after
24‑h incubation time. Statistical analysis was conducted using a one‑way analysis of
variance and post hoc least significant difference test. In qualitative analysis, the 4´,
6‑diamidino‑2‑phenylindole staining method was used. The evaluation has shown a
significant result when 250, 300, and 350 μmol/L concentration of L‑arginine amino acid
culture media compared with control, and 400 μmol/L has the best result and was not
significantly different with control toward viability of hDPSCs.
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