000			a
999			_c11063 _d11063
003			OSt
005			20200206111604.0
008			200206b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_911938 _aSirajudheen Anwar
245			_aIn vivo anticlastogenic effect of silymarin from milk thistle Silybum marianum L
250			_aVol.50(3), May-June
260			_aMumbai _bWolter Kluwer _c2018
300			_a108-115p.
520			_aOBJECTIVE: Silymarin, extracted from the seeds of Silybummarianum L. (Milk thistle), is traditionally used for treating various illnesses such as diabetes, cancer, inflammation, hepatitis, liver cirrhosis, and renal problems. Acute cytotoxicity and genotoxicity studies have been reported with ambiguous outcomes; however, its relevant anticlastogenic potential is not yet evaluated. This study was aimed to evaluate in vivo subacute anticlastogenic properties of silymarin to validate its use as a medicinal agent.MATERIALS AND METHODS: Silymarin was isolated from seeds of milk thistle. Various genotoxicity bioassays of silymarin were performed using mice. First, the bone marrow cell proliferation was estimated by calculating mitotic index. Second, the chromosomal abnormalities in mice bone marrow cells were studied. Third, micronucleated polychromatic erythrocytes (MPE) test and in vivo activation of sister chromatid exchanges (SCEs) were carried out in mice bone marrow cells. Finally, primary spermatocytes were analyzed to estimate genotoxic effect of silymarin on germ cells.RESULTS: We found that silymarin is capable of inducing a significant increase (P ≤ 0.05) in cell proliferation of bone marrow cells. There is no increase in chromosomal aberrations following silymarin treatments. Results clearly showed that it significantly (P ≤ 0.05) decreased the MPE. Likewise, it was found to be a negative inducer of SCEs. It decreased in total abnormal metaphase, SCEs, MPE, and aberrant diakinesis.CONCLUSION: The results demonstrated that silymarin has a strong anticlastogenic activity upon mice genome in somatic and germ cells, indicating its safe use as a medicinal substance. Furthermore, it is not only safe but also has protective effect from clastogens
650		0	_94774 _aPHARMACOLOGY
700			_911939 _aMadkor, Hafez R.
773	0		_x0253-7613 _dAndheri - Mumbai Wolters Kluwer India Private Limited _tIndian Journal of Pharmacology
856			_uhttp://www.ijp-online.com/temp/IndianJPharmacol503108-2043425_054034.pdf _yClick here
942			_2ddc _cAR