000			a
999			_c13771 _d13771
003			OSt
005			20201207102058.0
008			201207b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_912688 _aZhang, D.
245			_aEffect of Danggui Sini Decoction on the Behaviour and Dorsal Root Ganglion TRP Channel of Neuropathic Pain in CCI Rats
250			_aVol.81(5), Sep-Oct
260			_aMumbai _bIndian Journal of Pharmaceutical Science _c2019
300			_a922-932p.
520			_aDanggui Sini decoction, a traditional Chinese medicine formula, has been extensively used as a remedy for neuropathic pain and other diseases in recent years. To explore whether Danggui Sini decoction can alleviate mechanical pain, thermal hyperalgesia, and cold pain at the same time, the role of temperature-sensitive transient receptor potential channel in neuropathic pain and the therapeutic effect of Danggui Sini decoction on neuropathic pain as well as the impact on the transient receptor potential pathway were explored in the present study. One hundred and twenty-eight Sprague Dawley rats were separated randomly into 4 groups; the sham-operated group, chronic constriction injury of sciatic nerve model group, pregabalin group, and Danggui Sini decoction-treated group. The rats in the sham-operated group underwent surgery, but no nerve was ligated. The rest of the rats were subjected to sciatic nerve ligation. Rats in Danggui Sinidecoction-treated group could be irrigated to Danggui Sini decoction. Rats in the sham-operated group and chronic constriction injury model group were gavaged with saline. Rats in pregabalin group were given pregabalin. Behavioural tests conducted were, acetone test and cold plate, hot plate experiment, and von Frey filaments on 4, 7, 14, 21st day, and then dorsal root ganglion neurons were taken out. Nerve ligation from the ipsilateral L4-6 dorsal root ganglion was measured TRPM8, TRPA1, TRPV1, TRPV2, TRPV3, and TRPV4 proteins expression by immunofluorescence staining and Western-blot method. Danggui Sini decoction could significantly inhibit the cold allodynia and the mechanical allodynia. The immunofluorescence studies showed that the model group of dorsal root ganglia on TRPM8, TRPA1, TRPV1, TRPV2, TRPV3, and TRPV4 proteins were decreased. Western-blot results showed the expression of TRPM8, TRPA1, TRPV1, TRPV2, TRPV3, and TRPV4 proteins in Danggui Sini decoction-treated group was reduced. Danggui Sinidecoction could not only suppress the cold allodynia, but also alleviate mechanical allodynia and thermal hyperalgesia. Danggui Sini decoction could down-regulate abnormally high expression of temperature-dependent transient receptor potential channel proteins.
650		0	_94639 _aPHARMACEUTICS
700			_912690 _aChen, D.
773	0		_tIndian journal of pharmaceutical sciences _x0250-474X _dNew Delhi Indian Pharmaceutical Association
856			_uhttps://www.ijpsonline.com/articles/effect-of-idanggui-sinii-decoction-on-the-behaviour-and-dorsal-root-ganglion-trp-channel-of-neuropathic-pain-in-cci-rats.pdf _yClick here
942			_2ddc _cAR