000			a
999			_c13815 _d13815
003			OSt
005			20201215102921.0
008			201215b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_912768 _aYuniarti, Erline
245			_aNatural Deep Eutectic Solvent Extraction and Evaluation of Caffeine and Chlorogenic Acid from Green Coffee Beans of Coffea canephora
250			_aVol.81(6), Nov-Dec
260			_aMumbai _bIndian Journal of Pharmaceutical Science _c2019
300			_a1062-1069p.
520			_aThis study describes the optimization of a method that involves the use of the natural deep eutectic solvents, choline chloride-sorbitol for extracting caffeine and chlorogenic acid from green coffee beans of Coffea canephora. Extensive screening of conditions based on response surface methodology, Box-Behnken design allowed establishing optimal extraction conditions as 4:1 for the mole ratio of choline chloride-sorbitol, 60 min of extraction time, and 1:30 g/ml for the liquid-solid ratio, which yielded 5.87 mg/g of caffeine and 12.24 mg/g of chlorogenic acid. In case of the latter compound, this constitutes an increase of 297 % compared to the conventional maceration method. The evaluation of the extract obtained under optimal conditions for lipase inhibitory activity revealed that the extract inhibited porcine pancreatic lipase, with an IC50 value of 32.46 μg/ml. The combination of a natural deep eutectic solvent with ultrasound-assisted extraction technology constituted the basis for the development of an environmentally friendly and easy-to-use extraction method.
650		0	_94639 _aPHARMACEUTICS
700			_912769 _aSaputri, Fadlina C.
773	0		_x0250-474X _dNew Delhi Indian Pharmaceutical Association _tIndian journal of pharmaceutical sciences
856			_uhttps://www.ijpsonline.com/articles/natural-deep-eutectic-solvent-extraction-and-evaluation-of-caffeine-and-chlorogenic-acid-from-green-coffee-beans-of-icof.pdf _yClick here
942			_2ddc _cAR