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_c15537 _d15537 |
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| 005 | 20211122121050.0 | ||
| 008 | 211122b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_914719 _a Mendoza, Ronel Abad |
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| 245 | _aBioassay-Guided Isolation and Structure Elucidation of Bioactive Phytoconstituents with Inhibitory Activity against Carbohydrate-Hydrolyzing Enzymes from the Aerial Parts of Premna odorata Blanco | ||
| 250 | _aVol.55(3), Jul-Sep | ||
| 260 |
_aBanagalore _bAssociation of Pharmaceutical Teachers of India (APTI) _c2021 |
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| 300 | _a846-856p. | ||
| 520 | _aBackground: This study aimed to isolate the bioactive components from the aerial parts of Premna odorata Blanco and to evaluate the hypoglycemic potentials of the crude extracts, sub-fractions and final isolate. Materials and Methods: The plant material underwent a series of enzyme assay-guided isolation and purification having the fractions assayed for inhibitory activity against α-amylase using 3,5-dinitrosalicylic acid (DNS) colorimetric method for each stage. H.1 isolate was tested for α-amylase and α-glucosidase inhibitory activity and glucose uptake by yeast cells. Phytochemical characterization and 1H and 13C NMR spectral analysis were done for the structure elucidation of H.1. Results: In vitro amylase studies revealed that at 100 μg/mL concentrations, hexane crude extract, fraction F (20%/80% and 10%/90% n-hexane/dichloromethane fractions) and F.3 (10:1 v/v petroleum ether/ethyl acetate sub-fraction) exhibited 34.38±0.116%, 71.86± 4.909% and 42.16±1.257% inhibition against α-amylase (at 1000 μg/mL concentration), respectively. H.1 isolate exerted significant inhibition (p < 0.05) of 55.99± 2.202% and 72.43±3.988% against α-amylase and α-glucosidase enzyme (both at 1000 μg/ mL concentration), respectively and significant glucose uptake of 13.85± 0.368%. The purified isolate was spectroscopically confirmed as a 5:1 mixture of β- sitosterol (H.1a) and stigmasterol (H.1b). Conclusion: The compounds have significant inhibitory activity against carbohydrate-hydrolyzing enzymes and may be potentially developed as adjuvant pharmacotherapy for type 2 diabetes. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 700 |
_914720 _aShen, Chien-Chang |
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| 773 | 0 |
_tIndian journal of pharmaceutical education and research _dBengluru Association of Pharmaceutical Teachers of India (APTI) _x0019-5464 |
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| 856 |
_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-55-3-846.pdf _yClick here |
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| 942 |
_2ddc _cAR |
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