| 000 | a | ||
|---|---|---|---|
| 999 |
_c15572 _d15572 |
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| 003 | OSt | ||
| 005 | 20211124094013.0 | ||
| 008 | 211124b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_914781 _aYan, Huan |
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| 245 | _aPunicalagin Suppresses LPS-induced Inflammatory Responses in Murine Macrophages via JAK/STAT Signaling Pathway and Zymosan-induced Mice Paw Edema | ||
| 250 | _aVol.55(2), Apr-Jun | ||
| 260 |
_aBanaglore _bAssociation of Pharmaceutical Teachers of India (APTI) _c2021 |
||
| 300 | _a550-555p. | ||
| 520 | _aAim: To investigate the anti-inflammatory activity of punicalagin (PUN) on mouse macrophages and the underlying mechanisms. Materials and Methods: Mouse primary peritoneal macrophages (PMs) were treated with lipopolysaccharide (LPS; 10μg/ml) and/ or PUN (50μM) for 4, 8 and 12 hr. The expression levels of inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, IL-6, IL-12P40 and CCL-2 mRNAs were analyzed by qRT-PCR. Total iNOS, MAPK and JAK/STAT protein levels were measured by Western blotting and the secreted levels of IL-1β, IL-6, IL-12P40 and IL-1270 by ELISA. Acute paw edema was induced in mice with zymosan and the animals were treated with high (50 mg/kg), medium (25 mg/kg) and low (12.5 mg/kg) doses of PUN. The levels of IL-1β, IL-6, CCL-2 and other related inflammatory factors and chemokines in the mouse paw tissue homogenates were detected by ELISA. Results: Compared to the LPS-treated cells, PUN significantly decreased the levels of inflammatory cytokines and other factors (P<0.05). In addition, high dose of PUN down regulated p-ERK, p-JAK1, p-STAT1 and p-STAT3, without significantly affecting p-p38 and p-JNK. Finally, acute paw edema in the mouse model was alleviated within 12h of high-dose PUN administration, which also inhibited inflammatory cytokines and chemokines. Conclusion: PUN inhibits macrophage-mediated inflammation and the secretion of inflammatory cytokines/chemokines by targeting the ERK/MAPK and JAK/STAT signaling pathways. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 700 |
_914782 _aGulimire, Abilaiti |
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| 773 | 0 |
_dBengluru Association of Pharmaceutical Teachers of India (APTI) _tIndian journal of pharmaceutical education and research _x0019-5464 |
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| 856 |
_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-55-2-550.pdf _yClick here |
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| 942 |
_2ddc _cAR |
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