000			a
999			_c15719 _d15719
003			OSt
005			20220107112939.0
008			211223b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_915021 _aNAGAMANI, J. E.
245			_aIn vitro study on protease and thrombolytic activity of aqueous extract from leucas aspera (L.) leaves
250			_aVol.13(7)
260			_aM P _bInnovare Academic Sciences Pvt Ltd _c2021
300			_a73-76p.
520			_aObjective: The current study is an attempt to screen for the in vitro clot lysis and proteolytic activity of aqueous extract of Leucas aspera leaves. Methods: Thrombolytic activity and protease activity of the crude enzyme obtained by ammonium sulfate precipitation and dialysis were assayed using blood clot and casein as substrates respectively. Native PAGE and gel documentation studies were performed to calculate the molecular weight of the enzyme. Results: In the study, 40% salt fractioned crude enzyme sample exhibited significant thrombolytic and caseinolytic activity. Further dose-dependent increased activity was observed with the maximum lytic activity of 52.11±1.04 % at 1 mg/ml of the sample when compared to the reference drug streptokinase (71.39±0.32%). Also, 68.72±0.62 U/hr of caseinolytic activity was observed for 1 mg/ml of the sample fraction. Conclusion: The study highlights and validates the efficacy of Leucas aspera leaves extract for thrombolytic and proteolytic actions. Enzyme with an approximate molecular weight, 19.89 KDa could be responsible for the significant lytic activity
650		0	_94639 _aPHARMACEUTICS
700			_915022 _a SAH, USHA
773	0		_x0019-5464 _tIndian journal of pharmaceutical education and research _dBengluru Association of Pharmaceutical Teachers of India (APTI)
856			_uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/41210/24967 _yClick here
942			_2ddc _cAR