000			a
999			_c15752 _d15752
003			OSt
005			20211224122923.0
008			211224b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_915080 _a XIE, JING
245			_aEffects of Ambroxol Hydrochloride on Lung Tissue Cell Apoptosis and Vascular Remodeling in Rats with Smoke Induced Chronic Obstructive Pulmonary Disease
250			_aVol.83(3), May-June
260			_aMumbai _bIndian Journal of Pharmaceutical Science _c2021
300			_a594-602p.
520			_aTo evaluate the effects of ambroxol hydrochloride on lung tissue cell apoptosis and vascular remodeling in rats with smoke induced chronic obstructive pulmonary disease. Sprague Dawley rats were randomly divided into normal, model, experimental and control groups (n=20). The rat model of chronic obstructive pulmonary disease was established by fumigation using Yan’an Cigarettes for 64 d. After fumigation, the experimental and control groups were subcutaneously injected with 5 ml of Ambroxol hydrochloride (20 mg/kg) and 5 ml of Bambuterol hydrochloride (20 mg/kg) respectively, while the normal and model groups were intraperitoneally injected with an equal dose of normal saline. Following drug intervention for 28 d, the pathological changes in lung tissues, vascular remodeling, lung tissue cell apoptosis, expressions of alpha-smooth muscle actin and vascular endothelial growth factor, as well as levels of caspase-3, B-cell lymphoma-2 and B-cell lymphoma-2 associated X protein were detected by hematoxylin and eosin staining, elastic Van Gieson staining, terminal deoxynucleotidyl transferase dUTP nick end labeling staining, immunohistochemical staining and western blotting, respectively. Compared with normal group, the damage of lung tissues was obvious, the medial thickness of pulmonary arterioles significantly increased, the degree of vascular muscularization, apoptosis rate and expressions of alpha-smooth muscle actin, vascular endothelial growth factor, caspase-3 and B-cell lymphoma-2 associated X protein rose and the expression of B-cell lymphoma-2 decreased in model group (p<0.05). Compared with model group, the damage of lung tissues was significantly improved, the medial thickness of pulmonary arterioles decreased, the degree of vascular muscularization, apoptosis rate and expressions of alpha-smooth muscle actin, vascular endothelial growth factor, caspase-3 and B-cell lymphoma-2 associated X protein reduced and the expression of B-cell lymphoma-2 rose in experimental and control groups (p<0.05). Ambroxol hydrochloride can inhibit the apoptosis of lung tissue cells and improve vascular remodeling, thereby protecting the lung tissues of chronic obstructive pulmonary disease rats.
650		0	_94639 _aPHARMACEUTICS
700			_915082 _a QIN, YEYU
773	0		_tIndian journal of pharmaceutical sciences _dNew Delhi
856			_uhttps://www.ijpsonline.com/articles/effects-of-ambroxol-hydrochloride-on-lung-tissue-cell-apoptosis-and-vascular-remodeling-in-rats-with-smoke-induced-chron.pdf _yClick here
942			_2ddc _cAR