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040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_915093 _aSAILOR, G. U.
245			_aDesign of Experiment Approach Based Formulation Optimization of Berberine Loaded Solid Lipid Nanoparticle for Antihyperlipidemic Activity
250			_aVol.83(2), March-April
260			_aMumbai _bIndian Journal of Pharmaceutical Science _c2021
300			_a204-218p.
520			_aBerberine is an isoquinoline alkaloid possesses multitude of biological e ffects. However, quaternary amine cation of berberine causes poor water solubility, resulting in low bioavailability which limits its pharmacological purpose. The aim of this study was to prepare and optimize berberine loaded solid lipid nanoparticle and to evaluate its pharmacokinetic and antihyperlipidemic activity. The solid lipid nanoparticles were prepared by solvent injection method and 3 2 full factorial design was used to study the e ffect of concentration of polyvinyl alcohol (X 1) and amount of lipid (X 2) on particle size (Y 1) and entrapment e fficiency (Y2). The formulation was optimized using desirability function and evaluated for physicochemical, morphological, in vitro drug release and in vivo pharmacokinetic study. In vivo antihyperlipidemic activity of the formulation was also studied using high fat diet induced hyperlipidemia model. The formulation optimized by validated experimental design comprise of 1 % w/v polyvinyl alcohol, 279 mg lipid (stearic acid) to achieve particle size of 395 nm with 82.44 % entrapment e fficiency. In vitro release study of berberine loaded solid lipid nanoparticle showed an initial burst release followed by slow and continuous release. Berberine loaded solid lipid nanoparticle also showed 4.13 folds improvement in relative bioavailability compared to Berberine suspension. Furthermore, Berberine loaded solid lipid nanoparticle ameliorate the levels of total cholesterol (-41 %), TG (-49 %), lipoprotein cholesterol-C (-80 %) and high-density lipoprotein cholesterol(+119 %) compared to hyperlipidemic control and also found to be better than pure drug. The prepared berberine loaded solid lipid nanoparticle are successful drug delivery system demonstrating its e ffectiveness in controlling hyperlipidemia due to the improved bioavailability of berberine.
650		0	_94639 _aPHARMACEUTICS
700			_915095 _a RAMANI, V. D.
773	0		_dNew Delhi _tIndian journal of pharmaceutical sciences
856			_uhttps://www.ijpsonline.com/articles/design-of-experiment-approach-based-formulation-optimization-of-berberine-loaded-solid-lipid-nanoparticle-for-antihyperl.pdf _yClick here
942			_2ddc _cAR