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040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_915096 _aYUKSEL, S. N.
245			_aEvaluation of Real Time Cell Proliferation, Anti- Inflammatory and Wound Healing Potential of Helenalin on HaCaT Keratinocytes Treated with Lipopolysaccharide Stimulated Monocytes
250			_aVol.83(2), March-April
260			_aMumbai _bIndian Journal of Pharmaceutical Science _c2021
300			_a219-229p.
520			_aArnica montana, of the family Asteraceae, is used for its anti-inflammatory and wound healing properties, especially for bruises, tissue injuries and other traumas. However, its cellular and molecular mechanisms are not yet fully known. Its main active ingredient is helenalin, a sesquiterpene lactone, known as one of the main active ingredients of Asteraceae species. The aim of this study was to investigate cell proliferation, wound healing and anti-in flammatory activity of helenalin on human keratinocyte cells treated with lipopolysaccharide stimulated human monocytic cell line. Human keratinocyte cells were treated with different helenalin concentrations (0.095-50μM) to determine toxic and nontoxic concentrations. Then, the effects of nontoxic (0.02 and 0.2μM) and toxic (2 μM) helenalin concentrations on human keratinocyte cell proliferation and migration were determined using the real time cell analysis system. The scratch assay was performed to determine wound healing activity and supernatants were collected to determine the e ffects of helenalin on cytokine levels of human keratinocyte cells under in flammatory and non-in flammatory conditions. Under inflammatory conditions, 0.02 and 0.2μM helenalin increased keratinocyte proliferation and induced the most wound healing and anti-in flammatory activity in human keratinocyte cells. Cell proliferation and migration, wound healing and anti-in flammatory effects were higher in cells exposed to 0.02 μM helenalin than 0.2 μM. Helenalin has been found to decrease the production of in flammatory cytokines, especially under in flammatory conditions and increase wound closure by increasing cell proliferation and migration.
650		0	_94639 _aPHARMACEUTICS
700			_915098 _aDIKMEN, M.
773	0		_tIndian journal of pharmaceutical sciences _dNew Delhi
856			_uhttps://www.ijpsonline.com/articles/evaluation-of-real-time-cell-proliferation-antiinflammatory-and-wound-healing-potential-of-helenalin-on-hacat-keratinocy.pdf _yClick here
942			_2ddc _cAR