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040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_915155 _a Tang, Yang
245			_aMelatonin inhibits oxidized low density lipoprotein induced proliferation and migration of vascular smooth muscle cells and its mechanism
250			_aVol.83(2), March-April
260			_aMumbai _bIndian Journal of Pharmaceutical Science _c2021
300			_a354-361p.
520			_aThe e ffect and molecular mechanism of melatonin on the vascular smooth muscle cells proliferation and vascular smooth muscle cells migration induced by oxidized low density lipoprotein was explored in this paper. The first scheme: induce vascular smooth muscle cells with series of oxidized low density lipoprotein concentrations for 48 h (0 μg/ml, 10 μg/ml, 20 μg/ml, 50 μg/ml and 100 μg/ml). The second scheme: 50 μg/ml oxidized low density lipoprotein was used to induce vascular smooth muscle cells s for 0 h, 6 h, 12 h, 24 h and 48 h. The third option: first induce vascular smooth muscle cells with 50 μg/ml oxidized low density lipoprotein for 24 h, then treat vascular smooth muscle cells with series of melatonin concentrations for 48 h (1 nmol/l, 1 μmol/l, 0.1 mmol/l, 1 mmol/l). Cell Counting Kit-8 method was used to detect cell proliferation. Cells were randomly divided into blank control group, melatonin group (0.1 mmol/l melatonin treatment vascular smooth muscle cells 48 h), oxidized low density lipoprotein group (vascular smooth muscle cells were induced by 50 μg/ml oxidized low density lipoprotein for 24 h), oxidized low density lipoprotein+melatonin group (50 μg/ml of oxidized low density lipoprotein induced vascular smooth muscle cells for 24 h and then treated vascular smooth muscle cells s with 0.1 mmol/l of melatonin for 48 h). After relative treatment, cell cycle was detected by flow cytometry and cell migration was detected by scratch test. Proliferating cell nuclear antigen, alpha smooth muscle actin, cyclin A and extracellular regulatory protein kinase were detected by immuno fluorescence staining and Western blot 1/2 (extracellular regulatory protein kinase 1/2) signal pathway related protein expression. Compared with the blank control group, vascular smooth muscle cells s treated with 50 μg/ml oxidized low density lipoprotein could signi ficantly induce cell proliferation. After 24 h of induction by oxidized low density lipoprotein (50 μg/ml), the number of cells in S phase and G1 phase was decreased signi ficantly and the number of cells in G2 phase was increased signi ficantly. Cell migration was promoted and proliferating cell nuclear antigen, cyclin A, Cyclina, p-extracellular regulatory protein kinase 1/2 protein expression was up-regulated, down regulating the p-p21 protein level. The di fference was statistically signi ficant (p<0.01). The melatonin with series concentrations (1 nmol/l, 1 μmol/l, 0.1 mmol/l and 1 mmol/l) could inhibit the proliferation of vascular smooth muscle cells induced by oxidized low density lipoprotein to varying degrees. After vascular smooth muscle cells treatment with oxidized low density lipoprotein (50 μg/ml) for 24 h and melatonin (0.1 mmol/l) for 48 h, the cells in G1 and S phase increased significantly, the cells in G2 phase decreased signi ficantly, the cell migration was inhibited, which were compared with oxidized low density lipoprotein oxidized low density lipoprotein group. The expression of proliferating cell nuclear antigen, cyclin A, Cyclina, p-extracellular regulatory protein kinase 1/2 protein was decreased and the expression of p-p21 protein was up-regulated, the di fference was statistically significant (p<0.01). Melatonin can significantly inhibit the proliferation and migration of vascular smooth muscle cells induced by oxidized low density lipoprotein and its mechanism may be related to activating p21 and blocking extracellular regulatory protein kinase 1/2 signaling pathway
650		0	_94639 _aPHARMACEUTICS
700			_915156 _a Liu, Chuan
773	0		_tIndian journal of pharmaceutical sciences _dNew Delhi
856			_uhttps://www.ijpsonline.com/articles/melatonin-inhibits-oxidized-low-density-lipoprotein-induced-proliferation-and-migration-of-vascular-smooth-muscle-cells-.pdf _yClick here
942			_2ddc _cAR