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| 005 | 20211231105444.0 | ||
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_aAIKTC-KRRC _cAIKTC-KRRC |
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_915276 _a Shendge, Anil Khushalrao |
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| 245 | _aEvaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma | ||
| 250 | _aVol.53(5), Sep-Oct | ||
| 260 |
_aMumbai _bWolter Kluwer _c2021 |
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| 300 | _a377-383p. | ||
| 520 | _aINTRODUCTION: The use of natural resources as medicines for cancer therapies has been described throughout history in the form of traditional medicines. However, many resources are still unidentified for their potent biological activities.Clerodendrum viscosum is a hill glory bower reported as a remedy against oxidative stress, skin diseases, and intestinal infections. MATERIALS AND METHODS: We have collected the C. viscosum leaves and used for the preparation of 70% methanolic extract (CVLME). Then, CVLME has been confirmed for anticancer properties on various cancer cell lines by evaluating cytotoxicity, cell cycle analysis, induction of ROS and apoptosis, and nuclear fragmentation. Further, the phytochemical analysis of CVLME was evaluated through high‑performance liquid chromatography. RESULTS: Cell proliferation assay revealed the selective cytotoxicity of CVLME against breast cancer cell line (MCF‑7). The FACS‑based cell cycle analysis showed increased subG1 (apoptosis) population dose dependently. Further, the apoptosis‑inducing effect of CVLME was confirmed by annexin staining. Flow cytometry and confocal microscopy revealed the selective ROS generation upon CVLME treatment. The confocal‑based morphological study also revealed condensed and fragmented nuclear structure in CVLME‑treated MCF‑7 cells. Phytochemical investigations further indicated the presence of tannic acid, catechin, rutin, and reserpine which might be the reason for the anticancer activity of CVLME. CONCLUSION: The above‑combined results revealed the anticancer effect of CVLME, which may be due to the selective induction of ROS in breast carcinoma | ||
| 650 | 0 |
_94774 _aPHARMACOLOGY |
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_915277 _aBasu, Tapasree |
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| 773 | 0 |
_dAndheri - Mumbai Wolters Kluwer India Private Limited _x0253-7613 _tIndian Journal of Pharmacology |
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| 856 |
_uhttps://www.ijp-online.com/temp/IndianJPharmacol535377-1925666_052056.pdf _yClick here |
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_2ddc _cAR |
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