| 000 | a | ||
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| 999 |
_c16259 _d16259 |
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| 003 | OSt | ||
| 005 | 20220208115904.0 | ||
| 008 | 220208b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_915922 _aAgbokponto, Janvier Engelbert |
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| 245 | _aLiquid chromatography tandem mass spectrometry determination method of bencycloquidium bromide: application to drug interaction study in human | ||
| 250 | _aVol.13(10) | ||
| 260 |
_aM P _bInnovare Academic Sciences Pvt Ltd _c2021 |
||
| 300 | _a43-46p. | ||
| 520 | _aObjective: This study was conducted to develop a sensitive and effective LC-MS/MS method for the determination of bencycloquidium bromide (BCQB) and its application in pharmacokinetic drug interaction study between BCQB and paroxetine. Methods: The chromatographic separation was performed on Hedera ODS-2 C18 column with a mobile phase consisted of acetonitrile-10 mmol/l ammonium acetate containing 0.2% acetic acid (33:67, v/v) at 550μl/min, and the plasma samples were processed using solid-phase extraction. The MS/MS transitions were m/z 330.2→ 142.0 for BCQB and m/z 344.2→ 156.1 for the I. S in positive ESI mode.Results: The validated method was linear over the concentration range of 2-1200 pg/ml with the correlation coefficient r2>0.998. The intra-and inter-batch precisions of the assay were lower than 8.2% and 9.1%, respectively. The lower limit of quantification (LLOQ) was 2 pg/ml. The stability data at different storage conditions of BCQB were within±5% RE. The mean AUC0-36 of BCQB was increased by approximately 33%, after the administration of BCQB alone and upon co-administration with paroxetine during the drug interaction study.Conclusion: The LC-MS/MS method validated in this study was robust, reproducible, accurate, precise and reliable and was successfully applied in the pharmacokinetic drug interaction studies. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
|
| 700 |
_915923 _aYemoa, Loconon Achille |
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| 773 | 0 |
_x2656-0097 _dBhopal Innovare Academic Sciences Pvt Ltd _tInternational journal of pharmacy and pharmaceutical science |
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| 856 |
_uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/43232/25489 _yClick here |
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| 942 |
_2ddc _cAR |
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