| 000 | a | ||
|---|---|---|---|
| 999 |
_c16269 _d16269 |
||
| 003 | OSt | ||
| 005 | 20220208140228.0 | ||
| 008 | 220208b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
||
| 100 |
_915939 _aAlvi, Syed N. |
||
| 245 | _aMeasurement of cortisol and cortisone in human saliva by UPLC-MS/MS | ||
| 250 | _aVol.13(10) | ||
| 260 |
_aM P _bInnovare Academic Sciences Pvt Ltd _c2021 |
||
| 300 | _a54-58p. | ||
| 520 | _aObjective: To develop and validate a simple and rapid assay for simultaneous measurement of cortisol and cortisone in human saliva by ultra-performance liquid chromatography-tandem mass spectrometry.Methods: Chromatographic analysis was performed on an Atlantis dC18 column (2.1 x 100 mm, 3 μm) using a mobile phase consisting of acetonitrile and 2 mmol ammonium-acetate (50:50, v; v) that was delivered at a flow rate of 0.3 ml/min. The eluents were monitored using electrospray ionization in the positive ion mode set at transition set of mass-to-charge (m/z): 363.11 → 121.00, 361.18 → 163.11, and 367.19 → 121.24 for cortisol, cortisone and internal standard (IS), respectively the method was validated for linearity, accuracy, precision, and recovery, according to international guidelines. Results: The retention times of cortisol, cortisone and internal were about 1.38, 1.43 and 1.38 min, respectively. Cortisol level and cortisone levelrelationship to the ratio of their respective peak-area to IS’s peak-area was linear (range of 0.5-100 ng/ml). Coefficients of variation and inaccuracy were, ≤9.9% and-0.3 to 6.9 for cortisol and ≤8.4 and-1.5 to 4.8 for cortisone, respectively. Extraction recoveries for cortisol, cortisone, and the IS were 90%, 94%, and 98%, respectively.Cortisol and cortisone stability was evaluated in processed saliva samples (stored at room temperature for 24 h) and unprocessed saliva samples (stored at room temperature for 24 h or at-20 °C for 10 w) and after 3 freeze-thaw cycles was ≥ 86%.Conclusion: The proposed method is simple, precise, and accurate for the rapid simultaneous measurement of cortisol and cortisone levels in saliva. The assay was successfully applied to determine levels of cortisol and cortisone in human saliva samples obtained from healthy volunteers. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
|
| 700 |
_915940 _aHammami, Muhammad M. |
||
| 773 | 0 |
_x2656-0097 _dBhopal Innovare Academic Sciences Pvt Ltd _tInternational journal of pharmacy and pharmaceutical science |
|
| 856 |
_uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/41829/25205 _yClick here |
||
| 942 |
_2ddc _cAR |
||