000			a
999			_c17617 _d17617
003			OSt
005			20220923095839.0
008			220923b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_918063 _aShanmugam, Kondeti Ramudu
245			_aEffect of ocimum sanctum in sodium fluoride (NaF) induced fluorosis in rats: a study with respect to antioxidant enzymes and fluorosis markers
250			_aVol.56(1), Jan-Mar
260			_aKarnataka _bAssociation of Pharmaceutical Teachers of India (APTI) _c2022
300			_a175-183p.
520			_aIntroduction: Fluoride contamination was observed in groundwater’s of India, Pakistan, China, Indonesia and several other countries. In India the states like Andhra Pradesh, Telangana, Rajasthan, Haryana etc are contaminated with fluoride. Approximately 68 million people are suffering from fluorosis in India. The current study was done to know the antioxidant effect, anti-fluorosis and free radical scavenging property of Ocimum sanctum (OS). Methods: Superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione (GSH), glutathione reductase (GR), glutathione-s-transferase (GST), catalase (CAT), malondialdehyde (MDA), alanine aminotransferase (AAT), alkaline phosphatase (ALKP), aspartate aminotransferase (AST) and flurosis markers phosphorus (P4 ) and calcium (Ca) are estimated in all groups. 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), hydroxyl radical, hydrogen peroxide (H 2O2 ), Gas chromatography–mass spectrometry (GC-MS) was analyzed in methanolic extract of Ocimum sanctum. Results: SOD, GR, GSH, GPx CAT, activities and Ca levels are decreased in sodium fluoride (NaF) intoxicated rats. Whereas MDA, ALKP AST, AAT, and P4 levels are increased in NaF rats. However, Ocimum phenolic fraction treatment normalized the antioxidant enzymes, hepatic markers and flurosis markers in NaF intoxicated rats. DPPH, H 2O2 and hydroxyl radical of ocimum posses potent free radical scavenging activities. GC – MS studies of Ocimum sanctum showed the presence of many bioactive compounds like Caryophyllene oxide, 2-(2-Propenyl)-m-anisidine, 2H-Pyan-2-one, 5,6-Dihydro-4-(2-methyl-3-methylene- 1-buten-4-yl etc. Further, histopathological observations prove that ocimum protects the hepatic tissue from NaF intoxication. Conclusion: Our study proves that Ocimum sanctum phenolic fraction protects the hepatic tissue from Na F induced fluorosis in rats. Key words: Ocimum sanctum, Antioxidant Enzymes, Fluorosis markers, Liver markers, GC-MS analysis, Rats.
650		0	_94774 _aPHARMACOLOGY
700			_918064 _aShanmugam, Bhasha
773	0		_dBengluru Association of Pharmaceutical Teachers of India (APTI) _tIndian journal of pharmaceutical education and research _x0019-5464
856			_uhttps://www.ijper.org/sites/default/files/IndJPhaEdRes-56-1-175.pdf _yClick here
942			_2ddc _cAR