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005 | 20221122143151.0 | ||
008 | 221122b xxu||||| |||| 00| 0 eng d | ||
040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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100 |
_919226 _aYang, Jingyan |
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245 | _aMechanism of long non-coding RNA MAGI2- antisense RNA 3 targeting microRNA-31-5p to regulate proliferation and metastasis of SiHa cells in cervical cancer | ||
250 | _aVol.84(2), Mar-Apr | ||
260 |
_aMumbai _bIndian Journal of Pharmaceutical Science _c2022 |
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300 | _a477-482p. | ||
520 | _aTo investigate the effect of long non coding RNA MAGI2-antisense RNA 3 on the proliferation, invasion and migration of cervical cancer cells and the underlying mechanism. SiHa cells were divided into plasmid cloning DNA group, plasmid cloning DNA-MAGI2-antisense RNA 3 group, anti-microRNA-negative control group, anti-microRNA-31-5p group, plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA- negative control group and plasmid cloning DNA-MAGI2-antisense RNA 3+microRNA-31-5p group; quantitative reverse transcription polymerase chain reaction was used to determine the messenger RNA expression levels of microRNA-31-5p and MAGI2-antisense RNA 3; cell viability was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay; the number of cell migration and invasion in each group was detected by Transwell assay; dual luciferase reporter assay was performed to detect the fluorescence activity. The expression levels of microRNA-31-5p were significantly increased and those of MAGI2-antisense RNA 3 messenger RNA were decreased in cervical cancer cell lines (p<0.05); overexpression of MAGI2-antisense RNA 3 or inhibition expression of microRNA-31-5p could suppress SiHa cell proliferation, migration and invasion (p<0.05). MAGI2-antisense RNA 3 targeted regulation of microRNA-31-5p; overexpression of microRNA-31-5p partially reversed the inhibitory effects of overexpressing MAGI2-antisense RNA 3 on the proliferation, migration, invasion of SiHa cells. Long non coding RNA MAGI2-antisense RNA 3 can inhibit cervical cancer cell proliferation, migration and invasion by regulating microRNA-31-5p. | ||
650 | 0 |
_94639 _aPHARMACEUTICS |
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700 |
_919227 _aChen, Jing |
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773 | 0 |
_dNew Delhi _tIndian journal of pharmaceutical sciences |
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856 |
_uhttps://www.ijpsonline.com/articles/mechanism-of-long-noncoding-rna-magi2antisense-rna-3-targeting-microrna315p-to-regulate-proliferation-and-metastasis-of-.pdf _yClick here |
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_2ddc _cAR |