000			a
999			_c20256 _d20256
003			OSt
005			20231207102837.0
008			231207b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_922316 _aReddy, Srinivasa
245			_aSimultaneous estimation of ampicillin and sulbactam in human plasma by liquid chromatography tandem mass spectrometry
250			_aVol.13(1), Jan-Mar
260			_aRaipur _bAsian Pharma Press _c2023
300			_a13-20p.
520			_aA LCMS/MS method for the simultaneous determination of ampicillin and sulbactam in human plasma was described. After protein precipitation using 2mL of acetonitrile, 250µL of supernatant was mixed with 1.000 mL of 0.1% Acetic Acid in Milli-Q-water. 10µL was injected to a Biobasic AX column and eluted with 10mM Ammonium acetate and Acetonitrile: 60:40, v/v at a flow rate of 0.5mL/min. MRM transitions were monitored in negative mode as m/z 348.1 ? 206.8 (AMP), 231.9 ? 187.8 (SUL) and m/z 353.0 ? 211.9 (AMP D5). Sample concentrations were calculated by linear regression analysis using the analyst software1.6.3. An excellent linear response was obtained over the concentration ranges 0.1040µg/mL to 10.1562µg/mL for Ampicillin and 0.0510µg/mL to 6.1552µg/mL for Sulbactam. The intra-day and inter-day precision were within 3.50% for all analytes. The assay accuracy was 96.27 –103.59 %. Mean recoveries were 84.51% and 98.54% for ampicillin and sulbactam, respectively. The limits of detections were 0.026µg/mL and 0.013µg/mL for ampicillin and sulbactam. This method was successfully used for a bioequivalence study.
650		0	_94639 _aPHARMACEUTICS
700			_922317 _aThomas, Licto
773	0		_dRaipur A & V Publications _x2231-5667 _tAsian journal of pharmaceutical analysis
856			_uhttps://ajpaonline.com/AbstractView.aspx?PID=2023-13-1-3 _yClick here
942			_2ddc _cAR