| 000 | a | ||
|---|---|---|---|
| 999 |
_c20347 _d20347 |
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| 003 | OSt | ||
| 005 | 20231218131402.0 | ||
| 008 | 231218b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_922453 _a Kaushal, Pooja |
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| 245 | _aValidated analytical method for multicomponent analysis of famotidine and ofloxacin in bulk drug and tablet formulation by using UV-visible spectrophotometer and RP-HPLC | ||
| 250 | _aVol.13(3), Jul-Sep | ||
| 260 |
_aRaipur _bAsian Pharma Press _c2023 |
||
| 300 | _a162-170p. | ||
| 520 | _aA simple, sensitive, accurate, precise, and reproducible UV- spectrophotometric method and RP-HPLC methods were developed and validated for the estimation of Famotidine and Ofloxacin in bulk drug and pharmaceutical formulation. The Linearity regression was detected and shows a good linear relationship; in the concentration range of 10-50µg/mL (R2 >0.9908) for famotidine and 10-50µg/mL (r2>0.9913) for ofloxacin. The UV – spectrophotometric estimation was carried out by the first-order derivative spectrophotometric method and absorbance were recorded at 273 and 280nm. Beers range were found to be 5-50µg/mL, respectively for both drugs while, correlation coefficient r2 > 0.9988 and 0.9941 for famotidine and ofloxacin. The isoabsorptive point was found to be 274nm in HPLC optimized mobile phase composition, potassium dihydrogen orthophosphate: methanol (60:40). Chromatographic condition consisted of mobile phase potassium dihydrogen orthophosphate buffer pH 2.3, methanol (60:40v/v), run time 30 min, C-18 column (ODS Hypersil) and flow rate 0.8mL/minute. The retention time for famotidine and ofloxacin were found to be 2.44 min, 7.99 min. respectively, and detection at ?max 274nm for both drugs (overlain spectra). The UV methods and RP-HPLC showed good reproducibility and recovery with the percent relative standard deviation (RSD) less than 5%. As per ICH guidelines, the developed method was validated for linearity, accuracy, precision, Sandell's sensitivity, and repeatability proving its utility in the estimation of famotidine and ofloxacin in house tablet formulation. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 700 |
_922454 _aKushawaha, Shiv Kumar |
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| 773 | 0 |
_tAsian journal of pharmaceutical analysis _x2231-5667 _dRaipur A & V Publications |
|
| 856 |
_uhttps://ajpaonline.com/AbstractView.aspx?PID=2023-13-3-2 _yClick here |
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| 942 |
_2ddc _cAR |
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