000			a
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005			20240118163628.0
008			240118b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_922824 _aLay, Sammy Henry
245			_aViability of human dental pulp stem cells: The potential of L‑arginine‑based culture media
250			_aVol.14(4), Oct-Dec
260			_aMumbai _bWolter Kluwer _c2023
300			_a306-310p.
520			_aDental pulp is built by proteins that have various roles in the biological process of pulp, such as structural protein, regulation protein, and catalytic protein. L‑arginine, an amino acid and one of the building blocks of proteins, regulates pro‑inflammatory and anti‑inflammatory activity. Therefore, L‑arginine‑based culture has potential to promote dental pulp regeneration. This study aimed to investigate the potential of L‑arginine‑based culture in improving the viability of human dental pulp stem cells (hDPSCs). We evaluated the viability of hDPSCs in culture media supplemented with different concentrations of L‑arginine amino acid (250, 300, 350, and 400 μmol/L) and Dulbecco’s Modified Eagle Medium plus fetal bovine serum 10% (control) using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay after 24‑h incubation time. Statistical analysis was conducted using a one‑way analysis of variance and post hoc least significant difference test. In qualitative analysis, the 4´, 6‑diamidino‑2‑phenylindole staining method was used. The evaluation has shown a significant result when 250, 300, and 350 μmol/L concentration of L‑arginine amino acid culture media compared with control, and 400 μmol/L has the best result and was not significantly different with control toward viability of hDPSCs.
650		0	_94639 _aPHARMACEUTICS
700			_922825 _aMargono, Anggraini
773	0		_tJournal of advanced pharmaceutical technology and research _x2231-4040
856			_uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10723171/pdf/JAPTR-14-306.pdf _yClick here
942			_2ddc _cAR